The production of a chitin-like exopolysaccharide (EPS) was optimized through experimental design methods, evaluating the influence of urea, phosphate, and glucose. Under optimized conditions, up to 1.51 g/L was produced and its physicochemical characteristics were evaluated by chromatography, NMR, and FTIR spectroscopy, and rheological techniques. The results showed a homogeneous EPS (Mw 4.9 × 10⁵ g mol⁻¹) composed of chitin, linear polymer of β-(1→4)-linked N-acetyl-d-glucosamine residues. The acetylation degree as determined by ¹³C CP-MAS NMR spectroscopy was over 90 %. The EPS biological activities, such as antioxidant effect and antitumor properties, were evaluated. To the best of our knowledge, this is the first study on the production of a new alternative of extracellular chitin-like polysaccharide with promising bioactive properties from the filamentous fungus M. alpina.

通过实验设计方法优化了甲壳素样胞外多糖（EPS）的生产，评估了尿素，磷酸盐和葡萄糖的影响。在优化的条件下，最高可生产1.51 g / L，并通过色谱，NMR和FTIR光谱以及流变学技术评估其理化特性。结果表明均匀的EPS（Mw为4.9×10 ⁵克摩尔^-1甲壳质组成），β-​​（1→4）的线性聚合物-连接的Ñ乙酰基d -葡糖胺残基。乙酰化度由¹³确定C CP-MAS NMR光谱超过90％。评价了EPS的生物活性，例如抗氧化作用和抗肿瘤性质。据我们所知，这是首次研究从丝状真菌高山被孢霉产生具有希望的生物活性的细胞外几丁质样多糖的替代品。