The binding affinity and thermodynamics of family 4 carbohydrate-binding module (CBM4), belonging to type B CBM, on model surfaces of cellulose nanocrystals (CNC) and nanofibrils (CNF) were investigated by quartz crystal microbalance with dissipation monitoring (QCM-D) technology in real-time at different temperatures. The thermodynamic parameters associated with the interaction, such as Gibbs free energy, enthalpy change, entropy change and heat capacity were obtained using the van’t Hoff analysis via a nonlinear parameter estimation. The results demonstrated CBM4 binds preferentially to both CNF and CNC, whereas the driving forces behind them were very different. The former was related to the hydrogen bonds formed in the CBM4 clefts, resulting in a favorable enthalpy but compensated by unfavorable entropy change; on the contrary, the latter was mainly driven by favorable entropy but compensated by unfavorable enthalpic change due to water rearrangement.

通过耗散监测（QCM-D）用石英晶体微天平研究了属于B型CBM的家族4碳水化合物结合模块（CBM4）在纤维素纳米晶体（CNC）和纳米原纤维（CNF）的模型表面上的结合亲和力和热力学。技术在不同温度下实时显示。与相互作用有关的热力学参数，例如吉布斯自由能，焓变，熵变和热容量，是通过van't Hoff分析通过非线性参数估计获得的。结果表明，CBM4优先结合CNF和CNC，而它们背后的驱动力却大不相同。前者与CBM4裂缝中形成的氢键有关，产生了有利的焓，但被不利的熵变化所补偿。反之，