This work studies microalgae production using urban primary wastewater as the only nutrient source in a pilot-scale thin-layer cascade photobioreactor (TLPBR). The experiments were carried out by operating the photobioreactor in semi-continuous mode at a constant dilution rate (D = 0.3 days⁻¹) uninterrupted over ten months. The TLPBR was effective at removing the main nutrients (nitrogen, phosphorus and chemical oxygen demand, COD) with biomass productivities ranging from 28.3 g·m⁻²·day⁻¹ to 47.3 g·m⁻²·day⁻¹ over the different seasons of the year. In addition, the evolution of the cells' photosynthetic efficiency (photosystem II) was also analysed. The removal of the main nutrients was correlated with the changes in the microbial-bacteria consortium existing in the culture system. These microbial fluctuations were correlated with the temperature and the solar irradiance impinging on the culture surface. The most abundant bacterial phyla were Proteobacteria and Bacteroidetes with the most dominant order being Rhodobacterales and Sphingomonadales, belonging to the Alphaproteobacteria class. Functional analysis of the relationship between bacterial populations, removal of major nutrients and the environmental parameters showed that the three main bacteria orders together (41% of the total) represent the main bacteria groups, which remained over the months tested; however, their abundance was affected by fluctuations, which might be explained by environmental variations and major nutrients such as the chemical oxygen demand (COD).

这项工作研究了使用城市一级废水作为中试规模的薄层级联光生物反应器（TLPBR）中唯一的营养来源的微藻生产。通过以半连续模式以恒定稀释率（D = 0.3 days ^-1）连续十个月操作光生物反应器来进行实验。TLPBR可有效去除生物量生产率从28.3 g·m ^-2 ·day ^-1到47.3 g·m ^-2 ·day ^-1的主要营养素（氮，磷和化学需氧量，COD）。在一年中的不同季节。此外，还分析了细胞光合作用效率的演变（光系统II）。主要营养物质的去除与培养系统中存在的微生物-细菌群落的变化相关。这些微生物波动与照射在培养表面上的温度和太阳辐射相关。最丰富的细菌门是Proteobacteria和Bacteroidetes，最主要的顺序是Rhodobacterales和Sphingomonadales，属于Alphaproteobacteria类。对细菌种群，主要营养物质去除和环境参数之间关系的功能分析表明，三个主要细菌顺序（占总数的41％）共同代表了主要细菌种类，在测试的几个月中仍然存在；但是，它们的丰度受到波动的影响，这可以用环境变化和主要营养物质（例如化学需氧量（COD））来解释。