Key message

Genome-wide identification of WD40-like genes reveals a duplication of COP1-like genes, one of the key players involved in regulation of flowering time and photomorphogenesis, with strong functional diversification in Rosaceae.

Abstract

WD40 proteins play crucial roles in a broad spectrum of developmental and physiological processes. Here, we conducted a systematic characterization of this family of genes in Rosa chinensis ‘Old Blush’ (OB), a founder genotype for modern rose domestication. We identified 187 rose WD40 genes and classified them into 5 clusters and 15 subfamilies with 11 of RcWD40s presumably generated via tandem duplication. We found RcWD40 genes were expressed differentially following stages of vegetative and reproductive development. We detected a duplication of CONSTITUTIVE PHOTOMORPHOGENIC1-like genes in rose (RcCOP1 and RcCOP1L) and other Rosaceae plants. Featuring a distinct expression pattern and a different profile of cis-regulatory-elements in the transcriptional regulatory regions, RcCOP1 seemed being evolutionarily conserved while RcCOP1L did not dimerize with RcHY5 and RcSPA4. Our data thus reveals a functional diversification of COP1-like genes in Rosacaeae plants, and provides a valuable resource to explore the potential function and evolution of WD40-like genes in Rosaceae plants.

中文翻译：

---

WD40 基因的全基因组鉴定揭示了蔷薇科 COP1 样基因的功能多样化。

关键信息

WD40 样基因的全基因组鉴定揭示了 COP1 样基因的重复，COP1 样基因是参与调控开花时间和光形态发生的关键参与者之一，在蔷薇科中具有很强的功能多样化。

抽象的

WD40 蛋白在广泛的发育和生理过程中发挥着至关重要的作用。在这里，我们对现代玫瑰驯化的创始人基因型Rosa chinensis 'Old Blush' (OB)中的这个基因家族进行了系统表征。我们鉴定了 187 个玫瑰WD40基因，并将它们分为 5 个簇和 15 个亚科，其中 11 个RcWD40可能是通过串联复制产生的。我们发现RcWD40基因在营养和生殖发育阶段后表达差异。我们在玫瑰中检测到重复的CONSTITUTIVE PHOTOMORPHOGENIC1 样基因（RcCOP1和RcCOP1L) 和其他蔷薇科植物。RcCOP1在转录调控区域具有独特的表达模式和不同的顺式调控元件谱，似乎在进化上是保守的，而 RcCOP1L 没有与 RcHY5 和 RcSPA4 二聚化。因此，我们的数据揭示了蔷薇科植物中COP1 样基因的功能多样化，并为探索蔷薇科植物中WD40 样基因的潜在功能和进化提供了宝贵的资源。