Farsetia macrantha Blatt. & Hallb. is an endemic and critically endangered plant species of Indian Thar desert. Being an endemic and threatened plant species, there is need to develop an efficient in vitro regeneration protocol for its conservation. Since, there is no published report on in vitro regeneration of F. macrantha, therefore, present study was designed to develop an efficient micropropagation method of F. macrantha along with foliar micromorphological studies. Cotyledonary node taken from 15 to 20 days old seedlings was used as an explant for in vitro regeneration. MS medium supplemented with additives ascorbic acid (50 mg l⁻¹), citric acid (25 mg l⁻¹), l-arginine (25 mg l⁻¹) and adenine sulphate (25 mg l⁻¹) and 0.5 mg l⁻¹ BA was best for shoot bud induction. Different types, concentrations and combinations of cytokinins (BA, Kin and mT) and auxin (IAA) tested for shoot multiplication, maximum number of shoots (25.10 ± 0.69) regenerated on MS medium containing additives and combination of two cytokinins 0.5 mg l⁻¹ BA + 0.25 mg l⁻¹ Kin. Microshoots were rooted successfully with highest rooting response (75.59%) on ½ MS medium containing 2.0 mg l⁻¹ IBA. In vitro rooted plants were hardened with ~ 75% survival rate. Comparative foliar micromorphological studies conducted among in vitro grown and hardened plants suggest the developmental changes of micropropagated plants during acclimatization. The present in vitro regeneration protocol could be used for large-scale propagation and conservation of F. macrantha.

Farsetia macrantha Blatt。＆Hallb。是印度塔尔沙漠的一种特有和极度濒危的植物。作为一种地方性和受威胁的植物物种，有必要开发一种有效的体外再生方案以保护其。由于目前尚无关于Mac。F. macrantha的体外再生的报道，因此，本研究旨在开发一种有效的F. Macrantha的微繁殖方法以及叶微形态学研究。从15到20天大的幼苗中取出的子叶节用作体外再生的外植体。MS培养基，添加了抗坏血酸（50 mg l ^-1），柠檬酸（25 mg l ^-1），1-精氨酸（25 mg l^-1）和硫酸腺嘌呤（25 mg l ^-1）和0.5 mg l ^-1 BA最适合诱导芽芽。测试了不同类型，浓度和组合的细胞分裂素（BA，Kin和m T）和生长素（IAA）的芽繁殖，在含有添加剂的MS培养基上再生了最多的芽数（25.10±0.69）和两种细胞分裂素0.5 mg l ^{− 1} BA + 0.25 mg l ^-1 Kin。在含有2.0 mg l ^-1的½MS培养基上以最高的生根响应（75.59％）成功生根了微芽。IBA。体外生根的植物变硬，存活率约为75％。在体外生长和变硬的植物之间进行的比较性叶微形态研究表明，适应环境下微繁植物的发育变化。本发明的体外再生方案可用于大规模繁殖和保存F. macrantha。