In 2015, some imported tangerine (Citrus reticulata Blanco) fruits with suspected canker lesions were observed in some local markets in Shiraz county of the Fars province in Iran. Symptomatic and asymptomatic tangerine fruits obtained in a survey of local markets were collected and analyzed. Xanthomonas-like bacterial isolates were only recovered from symptomatic tangerine fruits. Eighteen isolates were identified as Xanthomonas citri subsp. citri (Xcc) based on biochemical tests and polymerase chain reaction (PCR) using Xcc specific primers. The isolates were evaluated for pathogenicity on four Citrus spp., namely tangerine, sweet orange, lemon and Mexican lime. All isolates induced disease symptoms on the four Citrus spp. and could be re-isolated and identified from inoculated symptomatic plants. The pathogenicity test and a phylogenetic analysis of sequence data showed that Xcc pathotype A is the causal agent of bacterial canker in commercially imported tangerine fruits. Our results indicated that infected citrus fruits could play a relevant role as a dispersal inoculum of citrus canker into new regions such as Iran. To our knowledge, this is the first known introduction with imported fruits of Xcc pathotype A into the Fars province of Iran.

中文翻译：

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柑橘溃疡病病原体 Xanthomonas citri subsp. 的分离和表征。柑橘 A 型，发生在进口柑橘（Citrus reticulata Blanco）水果中

2015年，伊朗法尔斯省设拉子县部分市场出现疑似溃疡病的进口柑橘（Citrus reticulata Blanco）水果。收集并分析了在当地市场调查中获得的有症状和无症状的橘子水果。黄单胞菌样细菌分离株仅从有症状的橘子果实中回收。十八个分离株被鉴定为柑橘黄单胞菌亚种。citri (Xcc) 基于生化测试和聚合酶链反应 (PCR)，使用 Xcc 特异性引物。评估了分离株对四种柑橘属植物的致病性，即橘子、甜橙、柠檬和墨西哥酸橙。所有分离株均在四种柑橘属植物上诱发疾病症状。并且可以从接种的有症状的植物中重新分离和鉴定。致病性试验和序列数据的系统发育分析表明，Xcc 致病型 A 是商业进口橘子果实细菌性溃疡病的病原体。我们的研究结果表明，受感染的柑橘类水果可以作为柑橘溃疡病菌向伊朗等新地区的传播菌种发挥相关作用。据我们所知，这是第一个已知的将 Xcc 致病型 A 的进口水果引入伊朗法尔斯省。