At present, the traditional methods for the screening of Clostridium butyricum are not sufficiently selective and efficient. Therefore, it is necessary to establish a targeted and efficient screening method for the detection of C. butyricum. Bioinformatics was used in this study to find C. butyricum specific genes, and species-specific primers were designed based on the conserved regions of the targeted genes, followed by optimization of the PCR conditions. Methodological evaluation was carried out, and the results were compared with the traditional screening method based on Trypticase Sulfite Neomycin (TSN) selective medium. A high-efficiency PCR screening method, targeting C. butyricum species-specific primers, was established. The method was confirmed to have high specificity and sensitivity towards C. butyricum cut-off CFU 10³. Compared with the traditional method, the screening success rate of C. butyricum strains increased from 0.61 to 81.91%. The PCR screening method could quickly and accurately detect C. butyricum in samples and dramatically improve screening efficiency.

目前，传统的丁酸梭菌的筛选方法还没有足够的选择性和效率。因此，有必要建立有针对性和有效的筛选方法来检测丁酸梭菌。在这项研究中，使用了生物信息学技术来查找丁酸梭菌特异性基因，并根据靶基因的保守区域设计了种特异性引物，然后优化了PCR条件。进行了方法学评估，并将结果与​​基于胰蛋白酶亚硫酸盐新霉素（TSN）选择性培养基的传统筛选方法进行了比较。靶向丁酸梭菌的高效PCR筛选方法建立了物种特异性引物。证实该方法对丁酸梭菌截留CFU 10 ³具有高特异性和敏感性。与传统方法相比，丁酸梭菌菌株的筛选成功率从0.61提高到81.91％。PCR筛选方法可以快速，准确地检测样品中的丁酸梭菌，并大大提高筛选效率。