A Bacillus strain X2 that produced extracellular endo-xylanase (GH 11) (EC: 3.2.1.8) was isolated from the soil of the Northeast India region. This aerobic culture was Gram positive and endospore forming. Chemotaxonomic characterization showed variance with the fatty acid profile of related species in the Bacillus subtilis group. In Bacillus strain X2, distinct occurrence of iso-C_14:0 lipids is absent in other related species. The 16S rRNA gene sequence homology showed 99% similarity with Bacillus subtilis subsp. inaquosorum. The phylogenetic analysis by the multilocus sequence analysis (MLSA) of the nucleotide sequence of six concatenated genes (16S rRNA, groEL, gyrA, polC, purH and rpoB) resolved the taxonomic position of the Bacillus strain X2 in the Bacillus subtilis subsp. group. The MLSA showed that it is a member of a clade that includes Bacillus subtilis subsp. stercoris. In in silico DNA–DNA hybridization (DDH), the highest matching score was obtained with Bacillus subtilis subsp. stercoris (87%). The in silico DDH of the genome (G + C 43.7 mol %) shared 48.5%, with Bacillus subtilis subsp. inaquosorum. The MLSA phylogenetic tree and the highest degree of DNA hybridization, indicating that it belongs to the Bacillus subtilis subspecies stercoris.

从印度东北部地区的土壤中分离出产生胞外木聚糖内切酶 (GH 11) (EC: 3.2.1.8) 的芽孢杆菌菌株 X2。该需氧培养物为革兰氏阳性且形成内生孢子。化学分类学特征显示枯草芽孢杆菌组中相关物种的脂肪酸谱存在差异。在芽孢杆菌菌株 X2 中，其他相关物种中不存在明显的 iso-C _14:0脂质。 16S rRNA基因序列同源性与枯草芽孢杆菌亚种相似性达99%。伊纳夸索鲁姆。通过对六个串联基因（16S rRNA、 groEL 、 gyrA 、 polC 、 purH和rpoB ）的核苷酸序列进行多位点序列分析 (MLSA) 的系统发育分析，解析了芽孢杆菌菌株 X2 在枯草芽孢杆菌亚种中的分类位置。团体。 MLSA 显示它是包括枯草芽孢杆菌亚种在内的分支的成员。粪粪菌。在计算机DNA-DNA 杂交 (DDH) 中，枯草芽孢杆菌亚种获得了最高匹配分数。粪菌(87%)。基因组的计算机 DDH (G + C 43.7 mol %) 与枯草芽孢杆菌亚种共享 48.5%。伊那夸索鲁姆。 MLSA系统发育树和DNA杂交程度最高，表明其属于枯草芽孢杆菌粪类亚种。