Maternal effects contribute to adaptive significance for shaping various phenotypes of many traits. Potential implications of maternal effects are the cause of expression diversity, but these effects on mRNA expression and alternative splicing (AS) have not been fully elucidated in hybrid animals. Two reciprocal cross hybrids following hybridization of Megalobrama amblycephala (blunt snout bream, BSB) and Culter alburnus (topmouth culter, TC) were used as a model to investigate maternal effects. By comparing the expression of BSB- and TC- homoeologous genes between the two reciprocal cross hybrids, we identified 49–348 differentially expressed BSB-homoeologous genes and 54–354 differentially expressed TC-homoeologous genes. 2402, 2959, and 3418 AS events between the two reciprocal cross hybrids were detected in Illumina data of muscle, liver, and gonad, respectively. Moreover, 21,577 (TC-homoeologs) and 30,007 (BSB-homoeologs) AS events were found in the 20,131 homoeologous gene pairs of TBF3 based on PacBio data, while 30,561 (TC-homoeologs) and 30,305 (BSB-homoeologs) AS events were found in BTF3. These results further improve AS prediction at the homoeolog level. The various AS patterns in bmpr2a belonging to the bone morphogenetic protein family were selected as AS models to investigate the expression diversity and its potential effects to body shape traits. The distribution of differentially expressed genes and AS in BSB- and TC-subgenomes exhibited various changes between the two reciprocal cross hybrids, suggesting that maternal effects were the cause of expression diversity. These findings provide a novel insight into mRNA expression changes and AS under maternal effects in lower vertebrates.

中文翻译：

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母体效应决定了双子背叶××Culter alburnus的相互交叉杂交中父母等位基因的选择性剪接。

产妇效应有助于塑造许多性状的各种表型。母体效应的潜在影响是表达多样性的原因，但是在杂交动物中尚未完全阐明这些对mRNA表达和选择性剪接（AS）的影响。使用两个相互交叉的杂种杂交后，将Megalobrama amblycephala（钝嘴鲷，BSB）和Culter alburnus（topmouth culter，TC）杂交，作为研究母体效应的模型。通过比较两个相互交叉杂交的BSB-和TC-同源基因的表达，我们鉴定出49-348个差异表达的BSB同源基因和54-354个差异表达的TC同源基因。在肌肉，肝脏，肝脏，动物，动物和动物的Illumina数据中检测到两个相互交叉杂交的2402、2959和3418 AS事件。和性腺分别。此外，根据PacBio数据，在20,131个TBF3同源基因对中发现了21,577个（TC-同源）和30,007个（BSB-同源）AS事件，而发现了30,561个（TC-同源）和30,305（​​BSB-同源）AS事件。在BTF3中。这些结果进一步改善了同源物水平的AS预测。选择属于骨形态发生蛋白家族的bmpr2a中的各种AS模式作为AS模型，以研究表达多样性及其对身体形态特征的潜在影响。BSB和TC亚基因组中差异表达基因和AS的分布在两个相互交叉的杂种之间表现出各种变化，这表明母体效应是表达多样性的原因。