Gastric cancer is the fifth most common cancer worldwide. Along with environmental factors, such as Helicobacter pylori (H. pylori) infection, genetic changes play important roles in gastric tumor formations. miR-584 is a less well-characterized microRNA (miRNA), with apparent activity in human cancers. However, miR-584 expression pattern in gastric cancer development has remained unclear. This study aims to analyze the expression of miR-584 in gastric cancer samples and investigates the associations between this miRNA and H. pylori infection and clinical characteristics. The expression level of miR-584 was studied in primary gastric cancers versus healthy control gastric mucosa samples using the RT-qPCR method. The clinical data were analyzed statistically in terms of miR-584 expression. In silico studies were employed to study miR-584 more broadly in order to assess its expression and find new potential target genes. Both experimental and in silico studies showed up-regulation of miR-584 in patients with gastric cancer. This up-regulation seems to be induced by H. pylori infection since the infected samples showed increased levels of miR-584 expression. Deeper analyses revealed that miR-584 undergoes a dramatic down-regulation in late stages, invasive and lymph node-metastatic gastric tumors. Bioinformatics studies demonstrated that miR-584 has a substantial role in cancer pathways and has the potential to target STAT1 transcripts. Consistent with the inverse correlation between TCGA RNA-seq data of miR-584 and STAT1 transcripts, the qPCR analysis showed a significant negative correlation between these two RNAs in a set of clinical samples. miR-584 undergoes up-regulation in the stage of primary tumor formation; however, becomes down-regulated upon the progression of gastric cancer. These findings suggest the potential of miR-584 as a diagnostic or prognostic biomarker in gastric cancer.

中文翻译：

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miR-584在原发性和高级胃癌中的表达波动。

胃癌是全球第五大最常见的癌症。与环境因素（例如幽门螺杆菌（H. pylori）感染）一起，遗传变化在胃肿瘤形成中起重要作用。miR-584是特征性较差的microRNA（miRNA），在人类癌症中具有明显的活性。然而，miR-584在胃癌发展中的表达模式仍不清楚。这项研究旨在分析miR-584在胃癌样品中的表达，并研究该miRNA与幽门螺杆菌感染与临床特征之间的关系。使用RT-qPCR方法研究了miR-584在原发性胃癌与健康对照胃黏膜样品中的表达水平。根据miR-584表达对临床数据进行统计学分析。为了评估其表达并发现新的潜在靶标基因，使用了计算机研究来更广泛地研究miR-584。实验和计算机研究均显示胃癌患者中miR-584的上调。这种上调似乎是由幽门螺杆菌感染引起的，因为被感染的样品显示出增加的miR-584表达水平。更深入的分析显示，miR-584在晚期，侵袭性和淋巴结转移性胃肿瘤中经历了急剧的下调。生物信息学研究表明，miR-584在癌症途径中具有重要作用，并且具有靶向STAT1转录本的潜力。与miR-584的TCGA RNA-seq数据和STAT1转录本之间的逆相关性一致，qPCR分析显示一组临床样品中这两个RNA之间存在显着的负相关。miR-584在原发性肿瘤形成阶段经历上调；然而，随着胃癌的发展，它会被下调。这些发现表明miR-584作为胃癌诊断或预后生物标志物的潜力。