Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.,Food & Function

当前位置： X-MOL 学术 › Food Funct. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Antidiabetic effects and underlying mechanisms of anti-digestive dietary polysaccharides from Sargassum fusiforme in rats.
Food & Function ( IF 5.1 ) Pub Date : 2020-07-02 , DOI: 10.1039/d0fo01166e
Rui-Bo Jia ₁ , Zhao-Rong Li , Juan Wu , Zhi-Rong Ou , Baoguo Sun , Lianzhu Lin , Mouming Zhao

Affiliation

Sargassum fusiforme polysaccharides (SFP), an anti-digestive biologically active ingredient obtained from Sargassum fusiforme by ultrasound-assisted enzymatic extraction, have been proven to exhibit extremely strong alpha-glucosidase inhibitory activity. In the current research, the potential anti-diabetic effects and molecular mechanisms of SFP were investigated by classic biochemical analysis, high-throughput sequencing and molecular biology techniques in type 2 diabetic rats. The analysis of typical diabetic symptoms and serum profiles showed that oral administration of SFP could mitigate hyperglycemia, hyperinsulinemia, dyslipidemia and oxidative stress in diabetic rats. SFP also promoted glycogen synthesis in the liver and skeletal muscles. H&E staining observation confirmed that SFP intervention could partially repair liver and muscle injuries caused by diabetes. Moreover, 16S rRNA gene sequencing analysis indicated that SFP treatment could distinctly restore the beneficial composition of gut flora in diabetic rats. Furthermore, RT-qPCR analysis revealed that anti-diabetic effects of SFP may be closely related to accelerating the absorption and utilization of blood glucose in the liver and muscle and inhibiting hepatic glucose production. In short, this study demonstrated that SFP could be developed as functional foods or pharmaceutical supplements for the prevention or mitigation of diabetes and its complications.

中文翻译：

羊栖菜抗消化膳食多糖对大鼠的抗糖尿病作用和潜在机制。

羊栖菜多糖（SFP）是通过超声辅助酶法从羊栖菜中提取的一种抗消化生物活性成分，已被证明具有极强的α-葡萄糖苷酶抑制活性。目前的研究中，通过经典的生化分析、高通量测序和分子生物学技术在2型糖尿病大鼠中研究了SFP的潜在抗糖尿病作用和分子机制。对典型糖尿病症状和血清谱的分析表明，口服SFP可以减轻糖尿病大鼠的高血糖、高胰岛素血症、血脂异常和氧化应激。 SFP 还促进肝脏和骨骼肌中糖原的合成。 H&E染色观察证实SFP干预可以部分修复糖尿病引起的肝脏和肌肉损伤。此外，16S rRNA基因测序分析表明，SFP治疗可以明显恢复糖尿病大鼠肠道菌群的有益组成。此外，RT-qPCR分析表明，SFP的抗糖尿病作用可能与加速肝脏和肌肉对血糖的吸收和利用，抑制肝葡萄糖的产生密切相关。简而言之，这项研究表明，SFP 可以开发为功能性食品或药物补充剂，用于预防或减轻糖尿病及其并发症。

更新日期：2020-08-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11