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In Vivo Molecular Insights into Syntrophic Geobacter Aggregates.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-07-02 , DOI: 10.1021/acs.analchem.0c00653 Wenchao Wei 1, 2, 3 , Andrew Plymale 2 , Zihua Zhu 4 , Xiang Ma 5 , Fanghua Liu 1 , Xiao-Ying Yu 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-07-02 , DOI: 10.1021/acs.analchem.0c00653 Wenchao Wei 1, 2, 3 , Andrew Plymale 2 , Zihua Zhu 4 , Xiang Ma 5 , Fanghua Liu 1 , Xiao-Ying Yu 2
Affiliation
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Direct interspecies electron transfer (DIET) has been considered as a novel and highly efficient strategy in both natural anaerobic environments and artificial microbial fuel cells. A syntrophic model consisting of Geobacter metallireducens and Geobacter sulfurreducens was studied in this work. We conducted in vivo molecular mapping of the outer surface of the syntrophic community as the interface of nutrients and energy exchange. System for Analysis at the Liquid Vacuum Interface combined with time-of-flight secondary ion mass spectrometry was employed to capture the molecular distribution of syntrophic Geobacter communities in the living and hydrated state. Principal component analysis with selected peaks revealed that syntrophic Geobacter aggregates were well differentiated from other control samples, including syntrophic planktonic cells, pure cultured planktonic cells, and single population biofilms. Our in vivo imaging indicated that a unique molecular surface was formed. Specifically, aromatic amino acids, phosphatidylethanolamine components, and large water clusters were identified as key components that favored the DIET of syntrophic Geobacter aggregates. Moreover, the molecular changes in depths of the Geobacter aggregates were captured using dynamic depth profiling. Our findings shed new light on the interface components supporting electron transfer in syntrophic communities based on in vivo molecular imaging.
中文翻译：
体内对营养细菌的认识。
在自然厌氧环境和人工微生物燃料电池中，直接种间电子转移（DIET）被认为是一种新颖而高效的策略。在这项工作中研究了由金属还原土杆菌和还原性土细菌组成的营养模型。我们进行了营养分子和能量交换的界面的同养生物外表面的体内分子作图。被采用系统进行分析的液体的真空接口，具有时间飞行二次离子质谱法结合以捕捉互养的分子分布地杆菌在起居室和水合状态下的社区。通过选择峰进行主成分分析，发现共营养菌聚集体与其他对照样品（包括滑膜浮游细胞，纯培养的浮游细胞和单种群生物膜）有很好的区别。我们的体内成像表明形成了独特的分子表面。具体地，芳族氨基酸，磷脂酰乙醇胺部件和大的水分子簇被鉴定为一种有利于互养的DIET关键部件地杆菌聚集体。此外，使用动态深度分析技术可以捕获Geobacter聚集体深度的分子变化。我们的发现为基于体内分子成像的支持在同养菌群落中电子转移的界面成分提供了新的思路。
更新日期：2020-08-04
中文翻译：
体内对营养细菌的认识。
在自然厌氧环境和人工微生物燃料电池中，直接种间电子转移（DIET）被认为是一种新颖而高效的策略。在这项工作中研究了由金属还原土杆菌和还原性土细菌组成的营养模型。我们进行了营养分子和能量交换的界面的同养生物外表面的体内分子作图。被采用系统进行分析的液体的真空接口，具有时间飞行二次离子质谱法结合以捕捉互养的分子分布地杆菌在起居室和水合状态下的社区。通过选择峰进行主成分分析，发现共营养菌聚集体与其他对照样品（包括滑膜浮游细胞，纯培养的浮游细胞和单种群生物膜）有很好的区别。我们的体内成像表明形成了独特的分子表面。具体地，芳族氨基酸，磷脂酰乙醇胺部件和大的水分子簇被鉴定为一种有利于互养的DIET关键部件地杆菌聚集体。此外，使用动态深度分析技术可以捕获Geobacter聚集体深度的分子变化。我们的发现为基于体内分子成像的支持在同养菌群落中电子转移的界面成分提供了新的思路。
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