The genus Clostridium consists of a diverse group of pathogenic and non-pathogenic bacteria. The non-pathogenic clostridia contain several solventogenic members of industrial importance, such as Clostridium acetobutylicum or C. beijerinckii. In the process of acetone-butanol-ethanol (ABE) fermentation, these strains are used in large scale fermentation plants since almost 100 years. Soon after establishment of the first plants, the fermentation processes suffered from different bacteriophage infections worldwide. A limited set of studies addressing bacteriophages in solventogenic clostridia have been conducted since then. In this study, we present the genome sequence of the temperate bacteriophage TBP2 of the solventogenic strain C. saccharoperbutylacetonicum N1-4 (HMT) that is used for ABE fermentation. The phage genome consists of 38 039 bp and includes 48 open reading frames. Sequence analysis indicates that the genome encloses random parts of the bacterial genome in addition to its own DNA. It represents the first fully sequenced genome of a temperate bacteriophage infecting solventogenic clostridia.

中文翻译：

---

溶剂生产者 Clostridium saccharoperbutylacetonicum N1-4 (HMT, ATCC 27021) 的温和噬菌体 TBP2 的基因组序列分析。

属梭菌由一组不同的致病性和非致病性细菌的。非致病性梭菌包含几种具有工业重要性的产溶剂成员，例如丙酮丁醇梭菌或拜氏梭菌。在丙酮-丁醇-乙醇 (ABE) 发酵过程中，这些菌株用于大型发酵工厂已有近 100 年的历史。在第一批工厂建立后不久，发酵过程在世界范围内遭受了不同的噬菌体感染。从那时起，已经进行了一系列有限的研究来解决产溶剂梭菌中的噬菌体问题。在这项研究中，我们展示了产溶剂菌株的温带噬菌体 TBP2 的基因组序列用于 ABE 发酵的C. saccharoperbutylacetonicum N1-4 (HMT)。噬菌体基因组由 38 039 bp 组成，包括 48 个开放阅读框。序列分析表明，除了自身的 DNA 外，基因组还包含细菌基因组的随机部分。它代表了感染产溶剂梭菌的温带噬菌体的第一个完全测序的基因组。