Recently, Silver nanoparticles (AgNPs) have become widely applied nanomaterial in human contacting areas such as cosmetics, food and medicine due to their antibacterial property. On the other hand, surfactants are essential ingredient of several industrial and consumer formulations. Based on these important applications, the current research was aimed to carry out the synthesis and characterization of Tween 80 capped silver nanoparticles (T80-AgNPs) using gamma radiation reduction method. Characterization of T80-AgNPs was occurred by using UV–Vis, XRD, FTIR and TEM techniques. UV–Visible spectra showed surface plasmon resonance (SPR) peak in the range of 420 nm signifying the synthesis of colloidal AgNPs. TEM confirmed the formation of spherical and uniformly distributed AgNPs with average size of 18 nm. XRD analysis illustrated the formation of pure crystalline AgNPs. The FTIR analysis provides evidence for the stabilization of AgNPs by Tween 80. The synthesized T80-AgNPs were evaluated for antibacterial activity against both Escherichia coli (E. coli) as gram negative (G –ve) bacteria and Staphylococcus aureus (S. aureus) as gram positive (G + ve) bacteria and anti-biofilm activity to P. aeruginosa. The results show that T80-AgNPs exhibits excellent antibacterial and antibiofilm activities.

近来，由于银纳米颗粒（AgNPs）的抗菌特性，它们已在诸如化妆品，食品和药品等人接触领域中被广泛应用。另一方面，表面活性剂是几种工业和消费者配方中必不可少的成分。基于这些重要的应用，当前的研究旨在使用伽马射线还原法进行吐温80封端的银纳米颗粒（T80-AgNPs）的合成和表征。T80-AgNPs的表征是通过使用UV-Vis，XRD，FTIR和TEM技术进行的。紫外可见光谱显示在420 nm范围内的表面等离振子共振（SPR）峰表明了胶体AgNP的合成。TEM证实了球形和均匀分布的AgNP的形成，平均大小为18 nm。XRD分析表明形成纯结晶AgNP。FTIR分析提供了吐温80稳定AgNPs的证据。对合成的T80-AgNPs的抗菌活性进行了评估大肠杆菌（E. coli）为革兰氏阴性（G – ve）细菌，金黄色葡萄球菌（S. aureus）为革兰氏阳性（G + ve）细菌，并对铜绿假单胞菌具有抗生物膜活性。结果表明，T80-AgNPs具有优异的抗菌和生物膜活性。