ABSTRACT

CD55 and CD59 are complement regulatory proteins suggested to be related with progression of diabetes and its complications. The stromal cell-derived factor 1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR-4) are chemokine proteins. We aimed to investigate the relation of CD55 and CD59 expression levels and polymorphisms of SDF-1 and CXCR-4 with type 2 diabetes mellitus (T2DM) and its complications. Seventy-five T2DM patients and 73 controls were enrolled. Expression levels of CD55 and CD59 were measured by FACS Calibur; qRT-PCR was used to determine SDF-1 and CXCR-4 gene polymorphisms. CD55 and CD59 expressions in patients with nephropathy, retinopathy and cardiovascular disease were significantly lower than controls. Frequency of CXCR-4 T allele carrying was high in patients and created 1.6 fold risk for the disease (p = .07). CXCR-4 a allele carriers had decreased nephropathy; although there was no statistical significance in carrying CXCR-4 T allele, presence of nephropathy was approximately 2 times higher (p = .254). The nephropathy risk increased 10-fold in CXCR-4 TT genotype carriers (p = .02). All SDF-1 CC genotype carriers had retinopathy, so, it was considered that the CC genotype was effective in retinopathy development (p = .031). For the presence of cardiovascular disease, significant difference was observed for SDF-1 genotypes. Increased cardiovascular risk of 5- and 1.9-fold in SDF-1 T (p = .007) and CXCR-4 T (p = .216) allele carriers, respectively, was observed. We suggest that CD55 and CD59 protein levels and SDF-1 and CXCR-4 have predictive importance in process, complications and tendency of T2DM.

摘要

CD55 和 CD59 是补体调节蛋白，被认为与糖尿病及其并发症的进展有关。基质细胞衍生因子 1 (SDF-1) 和 CXC 趋化因子受体 4 (CXCR-4) 是趋化因子蛋白。我们旨在研究 CD55 和 CD59 表达水平以及 SDF-1 和 CXCR-4 多态性与 2 型糖尿病 (T2DM) 及其并发症的关系。招募了 75 名 T2DM 患者和 73 名对照者。CD55 和 CD59 的表达水平通过 FACS Calibur 测量；qRT-PCR 用于确定 SDF-1 和 CXCR-4 基因多态性。CD55和CD59在肾病、视网膜病和心血管疾病患者中的表达显着低于对照组。患者携带 CXCR-4 T 等位基因的频率很高，导致该疾病的风险增加了 1.6 倍。p = .07）。CXCR-4 a 等位基因携带者肾病减少；尽管携带 CXCR-4 T 等位基因没有统计学意义，但肾病的发生率大约高出 2 倍 ( p = .254)。CXCR-4 TT 基因型携带者的肾病风险增加了 10 倍 ( p = .02)。所有 SDF-1 CC 基因型携带者都有视网膜病变，因此认为 CC 基因型对视网膜病变的发展有效 ( p = .031)。对于心血管疾病的存在，观察到 SDF-1 基因型的显着差异。SDF-1 T ( p = .007) 和 CXCR-4 T ( p= .216) 分别观察到等位基因携带者。我们认为 CD55 和 CD59 蛋白水平以及 SDF-1 和 CXCR-4 在 T2DM 的过程、并发症和趋势中具有预测重要性。