The emergence of intestinal organoids, as a stem cell-based self-renewable model system, has led to many studies on intestinal development and cell-cell signaling. However, potential issues regarding the phenotypic stability and reproducibility of the methodology during culture still needs to be addressed for different organoids. Here we investigated the transcriptomes of jejunum organoids derived from the same pig as well as batch-to-batch variation of organoids derived from different pigs over long-term passage. The set of genes expressed in organoids closely resembled that of the tissue of origin, including small intestine specific genes, for at least 17 passages. Minor differences in gene expression were observed between individual organoid cultures. In contrast, most small intestine-specific genes were not expressed in the jejunum cell line IPEC-J2, which also showed gene expression consistent with cancer phenotypes. We conclude that intestinal organoids provide a robust and stable model for translational research with clear advantages over transformed cells.

作为基于干细胞的自我更新模型系统的肠道类器官的出现，导致了许多关于肠道发育和细胞信号传导的研究。但是，对于不同的类器官，仍然需要解决有关培养过程中方法的表型稳定性和可重复性的潜在问题。在这里，我们调查了同一只猪的空肠类器官的转录组，以及长期传代的不同猪类的类器官的批次间变化。在类器官中表达的基因集至少有17代与原始组织的基因集非常相似，包括小肠特异性基因。在各个类器官培养物之间观察到基因表达的微小差异。相反，空肠细胞系IPEC-J2中未表达大多数小肠特异性基因，该基因表达与癌症表型一致。我们得出的结论是，肠道类器官为转化研究提供了一个强大而稳定的模型，与转化细胞相比具有明显的优势。