The main goal of the study was to determine the biodiversity of bacteria and their metabolic profile in the rhizosphere of four winter wheat (Triticum aestivum L.) varieties (Hondia, Nordkap, Rotax, Tytanika) cultivated in Haplic Podzol soil in a no-tillage system. Two techniques, i.e., next generation sequencing (NGS, meta-barcoding of 16S rRNA community) and community level physiological profiling (CLPP), were applied to obtain a holistic picture of biodiversity. The basic soil chemical parameters (acidity, redox potential, carbon content, forms of nitrogen, and phosphorus) were also determined. It was found that the rhizospheric microbiome (at the genus level) of cv. Hondia and Rotax were significantly different from that present in the other cultivars studied. The CLPP technique demonstrated that microbial metabolic activity depended on both the type of substrate and wheat cultivars. Carbohydrates and carboxylic acids were the most easily utilized compounds in all rhizospheric soils. The principal component analysis (PCA) evidenced that the rhizospheric soils of Rotax and Nordkap were characterized by a higher functional activity (strong correlation with the Shannon-Wiener index, the Richness index, and utilization of hydrocarbons) than those of Hondia and Tytanika.

中文翻译：

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选定冬小麦（Triticum aestivum L.）品种根际的生物多样性—遗传和分解代谢指纹图谱

该研究的主要目标是确定细菌的生物多样性和四个小麦根际的代谢轮廓（小麦L.）品种（Hondia，Nordkap，罗塔克斯，Tytanika）栽培于触觉部灰壤免耕系统中的土壤。应用了两种技术，即下一代测序（NGS，16S rRNA社区的元条形码）和社区级生理概况（CLPP），以获得生物多样性的整体图景。还确定了土壤的基本化学参数（酸度，氧化还原电势，碳含量，氮和磷的形式）。发现cv的根际微生物组（属水平）。洪迪亚（Hondia）和罗塔克斯（Rotax）与其他研究品种存在显着差异。CLPP技术表明微生物的代谢活性取决于底物类型和小麦品种。碳水化合物和羧酸是所有根际土壤中最容易使用的化合物。