Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020),Small

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Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020)
Small ( IF 13.0 ) Pub Date : 2020-07-02 , DOI: 10.1002/smll.202070144
Prithvijit Mukherjee _{1,

2} , Eric J. Berns ₃ , Cesar A. Patino ₁ , Elamar Hakim Moully ₄ , Lingqian Chang ₁ , S. Shiva P. Nathamgari _{1,

2} , John A. Kessler ₅ , Milan Mrksich _{3,

4,

6} , Horacio D. Espinosa _{1,

2}

Affiliation

In article number 2000584, Milan Mrksich, Horacio D. Espinosa, and co‐workers report on a method for non‐destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction chambers, where they modify immobilized substrates on a self‐assembled monolayer, and their activity is quantified using self‐assembled monolayers for matrix‐assisted laser desorption/ionization (SAMDI) mass spectrometry.

中文翻译：

酶采样：通过局部电穿孔对活细胞中的酶进行时间采样，并通过SAMDI质谱法对活性进行定量（Small 26/2020）

Milan Mrksich，Horacio D. Espinosa及其同事在编号为2000584的文章中报告了一种使用微流活细胞分析设备中的局部电穿孔从小细胞群体多次无损采样酶的方法。这些酶被捕获在提取室中，在那里它们修饰固定在自组装单层上的底物，并使用自组装单层对基质辅助的激光解吸/电离（SAMDI）质谱进行定量。

更新日期：2020-07-02

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