The first outbreak of Zika virus (ZIKV) infection in the Americas, especially in Brazil, was reported in 2015. Fever, headache, rash, and conjunctivitis are the common symptoms of ZIKV infection. Unexpected clinical outcomes, such as microcephaly and Guillain-Barré syndrome, have also been reported. The recent spread of ZIKV and its association with severe illness has created an urgent need to understand its pathogenesis and find potential therapeutic targets. Studies show that some viruses, including Flavivirus, trigger oxidative stress, which affects cellular metabolism, viral cycle, and pathogenesis. However, the role of oxidative stress in ZIKV infection needs to be investigated. Here, we analyzed ZIKV infection-triggered oxidative stress and modified antioxidant enzyme activities. U87-MG and HepG2 cells were infected to measure reactive oxygen species (ROS), malondialdehyde (MDA), and carbonyl protein levels, the activities of superoxide dismutase (SOD) and catalase (CAT), and the activation of nuclear factor erythroid 2p45-related factor 2 (Nrf2). ZIKV infection induced a significant increase in ROS, lipid peroxidation, and protein carbonylation products and a significant decrease in SOD and CAT activities accompanied by inhibition of Nrf2 activation in both cell lines. Further, MDA and carbonyl protein levels and SOD and CAT activities were evaluated in the brain and liver of ZIKV-infected C57BL/6 mice, and oxidative stress associated with antioxidant depletion was also found to occur in vivo. Together, our findings indicate the potential use of antioxidants as a novel therapeutic approach to Zika disease, and future studies in this direction are warranted.

2015年，美洲（尤其是巴西）首次爆发寨卡病毒（ZIKV）感染。发烧，头痛，皮疹和结膜炎是ZIKV感染的常见症状。也已经报道了意外的临床结果，例如小头畸形和格林-巴利综合征。ZIKV的近来传播及其与严重疾病的关系迫切需要了解其发病机理并寻找潜在的治疗靶标。研究表明，某些病毒，包括黄病毒，触发氧化应激，从而影响细胞代谢，病毒循环和发病机理。但是，氧化应激在ZIKV感染中的作用有待研究。在这里，我们分析了ZIKV感染触发的氧化应激和修饰的抗氧化酶活性。感染U87-MG和HepG2细胞以测量活性氧（ROS），丙二醛（MDA）和羰基蛋白水平，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性以及核因子类红细胞2p45-相关因子2（Nrf2）。ZIKV感染引起ROS，脂质过氧化和蛋白质羰基化产物的显着增加，以及SOD和CAT活性的显着下降，同时抑制了两种细胞系中的Nrf2活化。进一步，体内。在一起，我们的发现表明抗氧化剂作为寨卡氏病的一种新颖治疗方法的潜在用途，因此有必要在此方向上进行进一步的研究。