Pollution due to heavy metal ions, including mercury, has become a major issue because of their toxicities. It is required to monitor mercury levels in aqueous media using fast and selective methods with high accuracy. Ellipsometry is a promising technique for instance when it's combined with the plasmon resonance phenomena. We reported a biosensor system available for qualitative/quantitative determination of mercuric ions in aqueous media where both the spectrophotometric ellipsometry and oligonucleotide recognition elements were used. A single step assay using both a linear (ProbeL) and a hair-pin (ProbeH) type oligonucleotide probe as a recognition element, in addition to a sandwich-type (ProbeLS) assay were developed and compared. The detection limits were 0.23 nM, 0.03 nM and 0.15 pM for ProbeL, ProbeH and ProbeLS, respectively. The detection range was between 0.05 nM and 100 nM Hg²⁺ for all assays proposed herein.

由于重金属离子（包括汞）的毒性，其污染已成为主要问题。需要使用高精度的快速选择性方法来监测水性介质中的汞含量。椭圆偏振法是一种很有前途的技术，例如当它与等离子体共振现象结合时。我们报道了一种生物传感器系统，可用于定性/定量测定水性介质中的汞离子，其中使用了分光光度椭圆偏振法和寡核苷酸识别元件。开发并比较了使用线性（ProbeL）和发夹（ProbeH）型寡核苷酸探针作为识别元件的单步测定法，并进行了比较。ProbeL，ProbeH和ProbeLS的检出限分别为0.23 nM，0.03 nM和0.15 pM。对于本文提出的所有测定，均为²⁺。