The detection of chlorogenic acid in clinic samples for metabolic kinetics study is quite a challenging task due to the low concentration and the lacking of sensitive quantitation methods. In this work, a porous pencil lead electrode (PLE) has been used to detect chlorogenic acid by square wave voltammetry (SWV) technique. The redox potential of chlorogenic acid is at ca. + 0.42V (pH = 3.0) versus saturated calomel electrode (SCE), and it’s a reversible thin layer electrochemical process with two-electrons and two-protons involved in this reaction. The sensitivity was significantly improved due to the enrichment of CGA at the porous structure of the electrode. Under the optimized conditions, the concentration of chlorogenic acid was linear in the range of 7.7 × 10^―8 ∼ 7.7 × 10^―6 M with detection limits down to 4.5 × 10^―9 M (R² = 0.9978, S/N = 3). The electrode was successfully used for the determination of chlorogenic acid in human urine with near 100 % recovery. Due to the advantages of simplicity of fabrication, high sensitivity, good reproducibility and easy renewability, we believe that the porous PLE is quite a promising candidate for determination of chlorogenic acid in clinic samples.

由于浓度低且缺乏灵敏的定量方法，因此在临床样本中检测代谢动力学研究中的绿原酸是一项艰巨的任务。在这项工作中，多孔铅笔铅电极（PLE）已用于通过方波伏安法（SWV）技术检测绿原酸。绿原酸的氧化还原电势约为。+ 0.42V（pH = 3.0）相对于饱和甘汞电极（SCE），这是一个可逆的薄层电化学过程，该过程涉及两个电子和两个质子。由于CGA在电极的多孔结构处富集，因此灵敏度得到了显着提高。在最佳条件下，绿原酸的浓度在7.7×10 ^―8范围内呈线性关系。约7.7×10 ^-6 M，检测极限低至4.5×10 ^-9 M（R ² = 0.9978，S / N = 3）。该电极已成功用于人类尿液中绿原酸的测定，回收率接近100％。由于制造简单，灵敏度高，可重复性好和易于更新的优点，我们认为多孔PLE是测定临床样品中绿原酸的很有希望的候选者。