Abstract Anthocyanin synthase (ANS), a key enzyme in anthocyanins synthesis, can convert the colorless leucoanthocyanins into colored anthocyanins. In this study, a potato anthocyanin synthase gene StANS was isolated and characterized. Expression analysis showed that the transcriptional expression of StANS in chromatic genotypes was significantly higher than that in yellow ones. Overexpressing the StANS gene could promote anthocyanin synthesis in potato tubers. Transient luciferase assays showed that the promoter of StANS could be activated by the transcription factors StAN2, StMYB113, and StAN11. These results indicated that the StANS gene, which was activated by the transcription factors StAN2, StMYB113, and StAN11, could promote the anthocyanins synthesis in potato tubers. These findings provide a theoretical and applicable basis for anthocyanins formation in colored potato tubers.

中文翻译：

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马铃薯花色苷合成酶基因StANS的功能分析

摘要 花青素合成酶（Anthocyanin synthase，ANS）是花青素合成的关键酶，可以将无色的无色花青素转化为有色的花青素。在这项研究中，分离并表征了马铃薯花青素合酶基因 StANS。表达分析表明StANS在染色基因型中的转录表达显着高于黄色基因型。过表达 StANS 基因可以促进马铃薯块茎中的花青素合成。瞬时荧光素酶分析表明，StANS 的启动子可以被转录因子 StAN2、StMYB113 和 StAN11 激活。这些结果表明，被转录因子StAN2、StMYB113和StAN11激活的StANS基因可以促进马铃薯块茎中花青素的合成。