Abstract Pericarp browning is one of the most important factors limiting the shelf life of litchi fruit. The storage behavior of three cultivars of litchi with different shelf life were studied to comprehensively address the importance of phenolic content and energy status in delaying the development of browning in litchi fruit after harvest. Results revealed that slower changes of browning indices; higher content of EGC, EC, ECG, GCG, PA2, total phenols and anthocyanins; lower activities of ANT, LAC, PPO, POD and PAL were detected in ‘Jingganghongnuo’, as compared to that in ‘Guiwei’ and ‘Nuomici’. The transcript abundant of oxidation-enzyme corresponding genes LcANT, LcLAC, LcPPO, LcPOD and LcPAL exhibited similar trends as changes of phenolics and enzyme activities in three cultivars. The energy status and the relative expression intensity of ATP metabolic-related genes LcATPb, LcSnRK2, LcAAC1, and LcAOX1 differed among the three cultivars and maintained higher levels in ‘Jingganghongnuo’. Accordingly, the development of pericarp browning was significantly related to the content of phenolics, especially ECG and EC, and to the changes in ATP of litchi fruit after harvest. The comparative study on variety of litchi cultivars evident that the phenolics, energy status as well as the transcript abundant of their corresponding genes are potential indicators to mark the browning change in litchi fruit.

中文翻译：

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酚类物质和能量状态对荔枝果实褐变和衰老的调控：三个不同品种的采后比较

摘要 果皮褐变是限制荔枝果实货架期的重要因素之一。对三个不同保质期荔枝品种的贮藏行为进行了研究，以综合探讨酚类含量和能量状态在延缓荔枝采收后褐变发展中的重要性。结果表明褐变指数变化较慢；EGC、EC、ECG、GCG、PA2、总酚和花青素含量较高；与'贵味'和'糯米'相比，'井冈红糯'中检测到的ANT、LAC、PPO、POD和PAL活性较低。氧化酶对应基因 LcANT、LcLAC、LcPPO、LcPOD 和 LcPAL 的转录丰度与三个品种中酚类物质和酶活性的变化表现出相似的趋势。ATP代谢相关基因LcATPb、LcSnRK2、LcAAC1和LcAOX1的能量状态和相对表达强度在3个品种间存在差异，并在'井冈红糯'中保持较高水平。因此，果皮褐变的发展与酚类物质，尤其是ECG和EC的含量，以及采后荔枝果实ATP的变化显着相关。荔枝品种的比较研究表明，其相应基因的酚类物质、能量状态以及转录本丰富程度是荔枝果实褐变变化的潜在指标。果皮褐变的发展与酚类物质，尤其是ECG和EC的含量，以及采后荔枝果实ATP的变化显着相关。荔枝品种的比较研究表明，其相应基因的酚类物质、能量状态以及转录本丰富程度是荔枝果实褐变变化的潜在指标。果皮褐变的发生与酚类物质，尤其是ECG和EC的含量，以及采后荔枝果实ATP的变化显着相关。荔枝品种的比较研究表明，其相应基因的酚类物质、能量状态以及转录本丰富程度是荔枝果实褐变变化的潜在指标。