Here, for the first time, a nanofibrous (NF) wound dressing comprising biomineralized polyacrylonitrile (PAN) nanofibers is developed. In contrast to the majority of the currently available nanofibrous wound dressings that are based on natural polymers, PAN is a synthetic, industrial polymer, rarely been considered for this purpose. PAN NFs are first hydrolyzed to allow for tethering of biofunctional agents (here Bovine Serum Albumin (BSA)). Later, the biofunctionlized PAN NFs are biomineralized by immersion in simulated body fluid (SBF). As a result, core-shell, calcium deficient hydroxyapatite (HA)/BSA/PAN nanofibers form, that are mechanically stronger (elastic modulus; 8.5 vs. 6 MPa) compared to the untreated PAN NFs. The biomineralized PAN NFs showed promising bioactivity as reflected in the cell biology tests with fibroblast and keratinocyte cells. Hs68 fibroblasts and HaCat keratinocytes were found to be more viable in the presence of the biomineralized NFs than when they were co-cultured with the neat PAN NFs. Such mechanical and biological characteristics of the biomineralized PAN NFs are favorable for wound dressing applications.

在这里，首次开发了包含生物矿化聚丙烯腈（PAN）纳米纤维的纳米纤维（NF）伤口敷料。与目前大多数基于天然聚合物的纳米纤维伤口敷料不同，PAN是一种合成的工业聚合物，很少为此目的被考虑。首先将PAN NFs水解以束缚生物功能剂（此处为牛血清白蛋白（BSA））。之后，将生物功能化的PAN NFs浸入模拟体液（SBF）中进行生物矿化。结果，形成了核壳，缺钙的羟基磷灰石（HA）/ BSA / PAN纳米纤维，与未处理的PAN NFs相比，其机械强度更高（弹性模量； 8.5对6 MPa）。生物矿化的PAN NFs具有良好的生物活性，这在成纤维细胞和角质形成细胞的细胞生物学测试中得到了反映。发现Hs68成纤维细胞和HaCat角质形成细胞在存在生物矿化NF的情况下比与纯PAN NF共同培养时更可行。生物矿化的PAN NF的这种机械和生物学特性对于伤口敷料应用是有利的。