The conventional preparation method of natural polymers is low in strength, which limits the application of multi stimuli responsive hydrogels as intelligent wound dressings. In this work, interpenetrating gelatin and γ-polyglutamic acid (γ-PGA) hydrogels with good biocompatibility, biodegradability and mechanical properties were prepared by hot-pressing pre-gelation of gelatin/γ-PGA and synergistic with gamma irradiation. The effects of radiation crosslinking dosage on gel fraction, swelling ratio, tensile strength and surface morphology were investigated. The biocompatibility of radiation cross-linked hydrogel was evaluated by aseptic test, hemolysis test, cytotoxicity test, delayed hypersensitivity reaction as well as in vivo degradation studies from in vitro to in vivo. The optimized hydrogel irradiated by 25 kGy has good water retention and biodegradability, especially the stimulation of temperature, pH value, salt species and concentration. The mechanical strength, biocompatibility and responsive properties of the hydrogel indicate that the intelligent hydrogel prepared by this method is a good hydrogel biomaterial for developing interactive wound dressing.

天然聚合物的常规制备方法强度低，这限制了多刺激响应水凝胶作为智能伤口敷料的应用。在这项工作中，通过热压明胶/γ-PGA的预胶凝并与γ射线协同作用，制备了具有良好生物相容性，生物降解性和机械性能的互穿明胶和γ-聚谷氨酸（γ-PGA）水凝胶。研究了辐射交联剂量对凝胶分数，溶胀率，拉伸强度和表面形态的影响。通过无菌试验，溶血试验，细胞毒性试验，迟发型超敏反应以及从体内到体内的体内降解研究，评价了辐射交联水凝胶的生物相容性。25 kGy辐照的优化水凝胶具有良好的保水性和生物降解性，尤其是对温度，pH值，盐类和浓度的刺激。水凝胶的机械强度，生物相容性和响应特性表明，用这种方法制备的智能水凝胶是开发交互式伤口敷料的良好水凝胶生物材料。