A real crude oil-contaminated soil was treated using a two-step method: biosurfactant-assisted soil washing and the biostimulated biotreating of the effluent. The mixture of surfactin and rhamnolipid could enhance the TPH removal from an oil-contaminated soil (32 g/kg) in the soil washing operation. 86% of TPH was removed from the oil-contaminated soil in the soil washing operation under the mixed biosurfactant (surfactin + rhamnolipid) of 0.6 g/L, the soil/water ratio of 20 w/v%, the temperature of 30 °C, and the washing time of 24 h, leaving an effluent containing 5028 mg/L TPH. The effluent was efficiently biotreated in the bioprocess with 5 g/L acclimate biomass daily stimulated with 0.1 mM H₂O₂, and the concentrtion of TPH decreased to 26 mg/L within 17 d corresponding a TPH biodegradation over 99%. The biostimulation with H₂O₂ caused the production of a high amount of peroxidase that could accelerate the biodegradation of TPH. Accordingly, the findings suggest that the biosurfactant-assisted washing operation combined with the H₂O₂-stimulated biodegradation process could be an enhanced green method for efficient treatment of the heavy oil-contaminated soils.

使用两步法处理受原油污染的真实土壤：生物表面活性剂辅助的土壤洗涤和废水的生物刺激性生物处理。表面活性素和鼠李糖脂的混合物可以在土壤洗涤操作中增强从油污土壤（32 g / kg）中去除TPH。在0.6 g / L的混合生物表面活性剂（表面活性素+鼠李糖脂），土壤与水的比例为20 w / v％，温度为30°C的条件下，通过土壤洗涤操作从油污土壤中去除了86％的TPH。 ，洗涤时间为24小时，剩下的废水中含有5028 mg / L TPH。废水在生物工艺中得到有效生物处理，每天用0.1 mM H ₂ O ₂刺激5 g / L的驯化生物质。，而TPH的浓度在17天之内降至26 mg / L，对应的TPH生物降解率超过99％。H ₂ O ₂的生物刺激引起大量的过氧化物酶的产生，可以加速TPH的生物降解。因此，这些发现表明，生物表面活性剂辅助的洗涤操作与H ₂ O ₂刺激的生物降解过程的结合可能是一种有效处理重油污染土壤的绿色方法。