The aim of this study was to assess the impact of the physiological state and intraspecific variability on the efficacy of 70% ethanol to inactivate conidia of Penicillium commune, used as a representative species of dairy product contaminants. Four physiological states were obtained by modifying the water activity during the production of conidia (0.995 and 0.950) and the harvesting conditions (hydrated and non-hydrated). These conditions were applied to four different P. commune strains isolated from contaminated dairy products. Five minutes exposure to 70% ethanol at ambient temperature allowed total inactivation of conidia (>4 log₁₀) regardless of the physiological state or the strain. For 1 min exposure, regardless of the strains, only dry-harvested conidia produced at a_w 0.950 exhibited survivors. Survival after 2 min exposure was observed for this physiological state for P. commune UBOCC-A-116003 only. For this strain, the impact of the physiological state was greater than 1.54 log₁₀ between dry-harvested conidia produced at a_w 0.950 that exhibited survivors after 1 min treatment and the 3 other kinds of conidia that were all inactivated. For 1 min exposure, by comparing the more resistant strain to the three other strains, the impact of the intraspecific variability was 2.35 log₁₀. These results demonstrated that the physiological state of the conidia, the representativeness of the tested species and strains should be taken into account to assess the efficacy of disinfectants in dairies.

这项研究的目的是评估生理状态和种内变异性对70％乙醇灭活青霉公社分生孢子的功效的影响，青霉公社被用作乳制品污染物的代表种类。通过改变分生孢子产生期间的水分活度（0.995和0.950）和收获条件（水合和非水合）获得四种生理状态。这些条件适用于从受污染的乳制品中分离出的四种不同的体育副杆菌。在环境温度下暴露于70％乙醇中五分钟使分生孢子完全失活（> 4 log ₁₀），而不考虑其生理状态或菌株。1次分钟曝光，在制作不管菌株的，仅干燥收获的分生孢子一个_瓦特0.950表现出幸存者。暴露2分钟后，仅对于P. com.une UBPCC-A-116003的这种生理状态观察到存活。对于该菌株，生理状态的影响在以0.950 _ww产生的干收获分生孢子在处理1分钟后表现出存活率，而其余3种均被灭活的分生孢子之间大于1.54 log ₁₀。对于1分钟的暴露，通过比较更具抵抗力的菌株与其他三个菌株，种内变异性的影响为2.35 log ₁₀。这些结果表明，应评估分生孢子的生理状态，被测物种和菌株的代表性，以评估乳制品中消毒剂的功效。