Abstract The effects of human milk oligosaccharides (HMOs) and the main component, 2′-fucosyllactose (2′-FL), on intestinal disorders were evaluated. A dextran sodium sulphate (DSS)-induced mice model for colitis was established to investigate the correlation between HMOs and 2′-FL in attenuating colitis and modulating gut microbiota. The results indicated that inflammatory bowel disease was attenuated by consumption of both HMOs and 2′-FL. Moreover, both dietary HMOs and 2′-FL inhibited the overgrowth of Streptococcus and Escherichia-Shigella. HMOs increased the proportion of Muribaculaceae and Bacteroides, and 2′-FL dramatically increased Lactobacillus abundance. This was positively correlated with short chain fatty acid production, but was negatively correlated with inflammatory biomarkers overall. PICRUSt analysis further showed that both HMO and 2′-FL regulated the bacterial genes related to infectious diseases and carbohydrate metabolism. Our findings suggested that 2′-FL had anti-inflammatory activities similar to those of HMOs; this might be related to regulation of the intestinal flora and metabolites.

中文翻译：

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2'-岩藻糖基乳糖补充剂对肠道炎性疾病小鼠肠道菌群的影响

摘要 评估了人乳寡糖 (HMO) 和主要成分 2'-岩藻糖基乳糖 (2'-FL) 对肠道疾病的影响。建立了葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型，以研究 HMOs 和 2'-FL 在减轻结肠炎和调节肠道微生物群方面的相关性。结果表明，同时食用 HMO 和 2'-FL 可减轻炎症性肠病。此外，膳食 HMO 和 2'-FL 均抑制链球菌和埃希氏菌-志贺氏菌的过度生长。HMOs 增加了 Muribaculaceae 和 Bacteroides 的比例，2'-FL 显着增加了乳酸菌丰度。这与短链脂肪酸的产生呈正相关，但与整体炎症生物标志物呈负相关。PICRUSt 分析进一步表明，HMO 和 2'-FL 均调节与传染病和碳水化合物代谢相关的细菌基因。我们的研究结果表明，2'-FL 具有与 HMO 相似的抗炎活性；这可能与肠道菌群和代谢物的调节有关。