Salmonella is one of the most common agents of foodborne disease worldwide. As natural alternatives to traditional antimicrobial agents, bacteriophages (phages) are emerging as highly effective biocontrol agents against Salmonella and other foodborne bacteria. Due to the high diversity within the Salmonella genus and emergence of drug resistant strains, improved efforts are necessary to find broad range and strictly lytic Salmonella phages for use in food biocontrol. Here, we describe the isolation and characterization of two Salmonella phages: ST-W77 isolated on S. Typhimurium and SE-W109 isolated on S. Enteritidis with extraordinary Salmonella specificity. Whole genome sequencing identified ST-W77 as a Myovirus within the Viunalikevirus genus and SE-W109 as a Siphovirus within the Jerseylikevirus genus. Infectivity studies using a panel of S. Typhimurium cell wall mutants revealed both phages require the lipopolysaccharide O-antigen, with SE-W109 also recognizing the flagella, during infection of Salmonella. A combination of both phages was capable of prolonged (one-week) antibacterial activity when added to milk or chicken meat contaminated with Salmonella. Due to their broad host ranges, strictly lytic lifestyles and lack of lysogeny-related genes or virulence genes in their genomes, ST-W77 and SE-W109 are ideal phages for further development as Salmonella biocontrol agents for food production.

沙门氏菌是全世界食源性疾病最常见的媒介之一。作为传统抗菌剂的天然替代品，噬菌体（噬菌体）作为对抗沙门氏菌和其他食源性细菌的高效生物防治剂正在出现。由于沙门氏菌属内的高度多样性和耐药菌株的出现，需要作出更大的努力来寻找广泛的，严格裂解的沙门氏菌噬菌体，以用于食品生物防治。在这里，我们描述了两种沙门氏菌噬菌体的分离和表征：S上分离的ST-W77 。鼠伤寒和SE-W109在S上分离。肠炎沙门氏菌特异性。全基因组测序确定ST-W77为Viunalikevirus属的一种肌病毒，而SE-W109为Jerseylikelike病毒的一种剑叶病毒。使用S面板进行传染性研究。鼠伤寒沙门氏菌细胞壁突变体显示，这两种噬菌体都需要脂多糖O抗原，而SE-W109在沙门氏菌感染期间也能识别鞭毛。当将两种噬菌体结合到受沙门氏菌污染的牛奶或鸡肉中时，它们能够具有延长的抗菌活性（一周）。由于它们的寄主范围广泛，严格的生活方式和基因组中缺乏溶原相关基因或毒力基因，ST-W77和SE-W109是理想的噬菌体，可作为沙门氏菌生物控制剂用于食品生产。