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Novel micro-straw for freezing small quantities of human spermatozoa
Fertility and Sterility ( IF 6.6 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.fertnstert.2020.03.032
Chuan Huang 1 , Run-Xin Gan 1 , Huan Zhang 1 , Wen-Jun Zhou 2 , Zeng-Hui Huang 3 , Su-Hua Jiang 2 , Xi-Ren Ji 2 , Fei Gong 3 , Li-Qing Fan 3 , Wen-Bing Zhu 3
Affiliation  

OBJECTIVE To evaluate a novel micro-straw as an efficient, simple method for freezing a small number of human spermatozoa for intracytoplasmic sperm injection (ICSI). DESIGN Prospective cohort study. SETTING Sperm bank. PATIENT(S) Men with severe oligozoospermia or azoospermia undergoing a total of 143 ICSI cycles at the CITIC-Xiangya Hospital of Reproduction and Genetics from June 1, 2015, to June 31, 2019, and 20 donors at the Hunan Province Human Sperm Bank from 2001 to 2016. INTERVENTION(S) Analysis of sperm samples and clinical outcomes after sperm use. MAIN OUTCOME MEASURE(S) Clinical information, including number of motile sperm before and after freezing, freeze-thaw survival rates, two-pronuclear fertilization rates, clinical pregnancy, and early pregnancy loss rates after sperm use. RESULT(S) In the feasibility experiment using the micro-straw, we found a freeze-thaw survival rate of 73% ± 8.3% and no difference in normal sperm morphology, normal acrosome integrity, or DNA fragmentation index between the micro-straw and 1.8-mL cryotubes. The prospective cohort included 1,325 cases, and we collected sperm from testicular, epididymis, and ejaculation sources. We observed motile sperm in 1,294 (97.6%) of 1,325 frozen-thawed samples. Postthaw sperm were available for ICSI in 140 (97.9%) of 143 of cycles. The fertilization, cleavage, and high-quality embryo rates were 1,007 (81.7%) of 1,233; 995 (98.8%) of 1,007; and 537 (53.9%) of 995, respectively. Sixty-nine (49%) clinical pregnancies were achieved, and the miscarriage rate was 6 (8.6%) of 69. CONCLUSION(S) The micro-straw is suitable and clinically useful for the cryopreservation of small numbers of spermatozoa.

中文翻译:

用于冷冻少量人类精子的新型微型吸管

目的 评估一种新型微型吸管作为一种有效、简单的方法,用于冷冻少量人类精子以进行卵胞浆内单精子注射 (ICSI)。设计前瞻性队列研究。设置精子库。PATIENT(S) 2015年6月1日至2019年6月31日在中信湘雅生殖遗传医院共接受143个ICSI周期的重度少精子症或无精子症男性,以及湖南省人类精子库的20名捐献者2001 年至 2016 年。 精子样本和精子使用后临床结果的干预措施。主要观察指标临床信息,包括冷冻前后活动精子数、冻融存活率、二原核受精率、临床妊娠、使用精子后早期流产率。结果在使用微型吸管的可行性实验中,我们发现微型吸管和微型吸管之间的冻融存活率为 73%±8.3%,并且正常精子形态、正常顶体完整性或 DNA 断裂指数没有差异。 1.8 毫升冷冻管。前瞻性队列包括 1,325 例病例,我们从睾丸、附睾和射精源收集精子。我们在 1,325 个冻融样本中的 1,294 个 (97.6%) 中观察到活动精子。在 143 个周期中的 140 个 (97.9%) 中,解冻后精子可用于 ICSI。受精、卵裂和优质胚胎率为 1,233 个中的 1,007 个(81.7%);995 (98.8%) 的 1,007;和 537 (53.9%) 的 995,分别。实现了 69 例 (49%) 临床妊娠,流产率为 69 例中的 6 例 (8.6%)。
更新日期:2020-08-01
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