Single-cell sequencing approaches offer exploration of tissue architecture at unprecedented resolution. These tools are especially powerful when deconvoluting highly specialized microenvironments, such as stem cell (SC) niches. Here, we review single-cell studies that map the cellular and transcriptional makeup of stem and progenitor niches and discuss how these high-resolution analyses fundamentally advance our understanding of how niche factors shape SC biology and activity. In-depth characterization of the blueprint of SC-niche crosstalk, as well as understanding how it becomes dysregulated, will undoubtedly inform the development of more efficient therapies for malignancies and other pathologies.

单细胞测序方法以前所未有的分辨率提供了组织结构的探索。当对高度专门化的微环境（例如干细胞（SC）壁ches）进行卷积处理时，这些工具特别强大。在这里，我们回顾了单细胞研究，这些研究描绘了茎和祖先壁的细胞和转录组成，并讨论了这些高分辨率分析如何从根本上增进我们对小生境因素如何影响SC生物学和活性的理解。毫无疑问，对SC利基串扰的蓝图的深入表征，以及对它如何失调的了解，无疑将为恶性肿瘤和其他病理学的更有效疗法的发展提供信息。