Chlorella sorokiniana FZU60, a lutein-enriching microalga, was cultivated in 50 L column photobioreactor to evaluate its potential for lutein production. Initial cell concentration, phosphate concentration and aeration rate were optimized, and results showed that optimal conditions of these three parameters were 0.10 g/L, 0.06 g/L and 0.02 vvm (2.5% CO₂), respectively. In addition, a novel two-stage strategy was successfully developed, in which algae were firstly cultivated under fed-batch mixotrophic condition to achieve high biomass concentration, and then shifted to photoautotrophic condition for enhancing lutein accumulation. Moreover, dissolved oxygen was found to be an efficient indicator of acetate depletion in fed-batch stage. The obtained lutein content, production and productivity reached 9.51 mg/g, 33.55 mg/L and 4.67 mg/L/d, respectively, which were greater than those reported in other pilot-scale studies. This proposed strategy provided a cost-effective approach for high-efficient microalgae-based lutein production at pilot-scale, indicating great potential for commercial production.

将富含叶黄素的微藻小球藻小球藻FZU60培养在50 L柱光生物反应器中，以评估其生产叶黄素的潜力。优化了初始细胞浓度，磷酸盐浓度和通气速率，结果表明这三个参数的最佳条件分别为0.10 g / L，0.06 g / L和0.02 vvm（2.5％CO ₂）， 分别。此外，成功地开发了一种新的两阶段策略，其中首先在补料分批混合营养条件下培养藻类以达到高生物量浓度，然后转移到光合营养条件下以增强叶黄素的积累。此外，发现溶解氧是补料分批阶段乙酸盐消耗的有效指标。获得的叶黄素含量，产量和生产率分别达到9.51 mg / g，33.55 mg / L和4.67 mg / L / d，高于其他中试规模研究中报道的水平。这项拟议的策略为中试规模的基于微藻的高效叶黄素生产提供了一种经济有效的方法，这表明了其商业生产的巨大潜力。