Abstract —The aim of this study was to investigate the protective effect of exogenous peroxiredoxins with ischemia–reperfusion of an isolated kidney. The study was carried out using a model of isolated rat kidney perfusion ex vivo. A recombinant peroxiredoxin 6 was injected directly in the perfusion buffer. The high-molecular-weight dye Blue Dextran 2000 and urea were added to the perfusion buffer to determine the functionality of an isolated kidney. It was demonstrated that exogenous peroxiredoxin 6 in the cortical layer of an isolated kidney was localized in the vessels of renal glomeruli; in the medulla it was found in microvessels surrounding the thin tubules. The use of peroxiredoxin 6 decreases the degree of damage of nephron structures by two times compared with the damage in the control, which provides the preservation of ultrafiltration processes. A decrease in glomerular damage leads to a decrease in the content of Blue Dextran by two times compared with the damage in the control at the end of the perfusion period. A decrease in the damage of tubular structures indicates the active urea transport during perfusion. Thus, the inclusion of peroxiredoxin 6 in the perfusion buffer mediated a decrease in the damage of nephron structures and maintenance of the multifunctional state of renal glomeruli and tubules.

中文翻译：

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外源性过氧还蛋白6对离体大鼠肾功能参数的影响

摘要 —本研究的目的是研究外源性过氧化物还原酶对离体肾脏缺血再灌注的保护作用。该研究是使用离体大鼠肾脏灌注模型进行的。将重组过氧还蛋白 6 直接注射到灌注缓冲液中。将高分子量染料 Blue Dextran 2000 和尿素添加到灌注缓冲液中，以确定离体肾脏的功能。研究表明，离体肾脏皮质层中的外源性过氧化物酶 6 定位于肾小球血管中；在髓质中，它存在于细管周围的微血管中。与对照组相比，使用过氧还蛋白 6 使肾单位结构的损伤程度降低了两倍，它提供了超滤过程的保存。与灌注期结束时的对照损伤相比，肾小球损伤的减少导致 Blue Dextran 的含量减少了两倍。管状结构损伤的减少表明灌注过程中尿素运输活跃。因此，在灌注缓冲液中加入过氧还蛋白 6 介导了肾单位结构损伤的减少和肾小球和肾小管多功能状态的维持。