Agaricus bisporus (Lange) Imbach is the most widely cultivated mushroom globally. Wet bubble disease (WBD), mainly caused by Mycogone perniciosa, is a major threat to A. bisporus production worldwide. In the current study, it was expected to select safe fungicides that can successfully control WBD on mushroom due to the biological similarity of A. bisporus and M. perniciosa. The in vitro sensitivity of 24 M. perniciosa isolates and five A. bisporus isolates to 26 different fungicides were tested. The control efficacies of 13 fungicides against WBD and their effects on A. bisporus were also determined in the field. The mean EC₅₀ values of the fungicides on M. perniciosa isolates ranged from 0.0020 to > 100 µg/ml (P < 0.05). Variation factor values suggested that the fungicides assayed had a narrow range of EC₅₀ values for M. perniciosa, with the exception of thiabendazole. In addition, five A. bisporus isolates exhibited highly variable sensitivities (mean EC₅₀ 0.0275 to > 100 µg/ml) to these fungicides. Apart from thiophanate-methyl and prochloraz-manganese, hymexazol, trifloxystrobin, fluazinam, fludioxonil, diniconazole, fenbuconazole, imazalil, mancozeb and iminoctadine trialbesilate showed good potential for the control of WBD in vitro sensitivity test. In field trials, prochloraz-manganese and thiabendazole continued to be useful to manage WBD and little effect on mushroom. Fludioxonil, diniconazole, imazalil and fenbuconazole at high doses exhibited better control of WBD and greater yields than other fungicides. And importantly, more rigorous fungicide application and novel cultural strategies are needed for management of WBD with high-quality A. bisporus.

双孢蘑菇（Lange）Imbach是全球种植最广泛的蘑菇。湿泡病（WBD）主要由多年生霉菌（Mycogone perniciosa）引起，是全球双孢曲霉生产的主要威胁。在目前的研究中，预计将选择安全杀菌剂，可以成功地控制WBD蘑菇由于生物相似性双孢蘑菇和M. perniciosa。测试了24种多年生青霉菌菌株和5种双孢曲霉菌株对26种不同杀菌剂的体外敏感性。13种杀菌剂对WBD的防治效果及其对双孢蘑菇的影响在现场也被确定。多年生芽孢杆菌分离物上杀菌剂的平均EC ₅₀值为0.0020至> 100 µg / ml（P <  0.05）。变化系数的值表明，测定的杀真菌剂具有EC的窄范围₅₀为值M. perniciosa，用噻苯咪唑除外。此外，五种双孢曲霉分离物对这些杀菌剂表现出高度可变的敏感性（平均EC ₅₀ 0.0275至> 100 µg / ml）。除甲基托布津酸和丙草胺锰外，羟甲唑，三氟羟雌黄酮，氟济南，氟地西尼，迪尼康唑，芬布康唑，咪唑，曼考布布和伊马卡定三氯苯磺酸盐显示出良好的控制WBD的潜力。体外敏感性测试。在田间试验中，原花青素锰和噻菌灵仍然对控制WBD有用，对蘑菇的影响很小。与其他杀菌剂相比，高剂量的氟二恶尼，迪尼康唑，咪唑和芬布康唑表现出更好的WBD控制和更高的产量。重要的是，需要管理更严格的杀菌剂和新颖的文化策略，以管理具有高质量双孢蘑菇的WBD 。