Herein, we report a novel fluorescent probe, hexaphenyl-1,3-butadiene derivative (ZZ-HPB-NC), with an aggregation-induced emission (AIE) feature, which shows a simple, highly selective, specific, and instant response to hepatocellular carcinoma (HCC). The fluorescence emission of ZZ-HPB-NC can be quickly turned-on in the intraoperative frozen-section slides of HCC specimens via a simple staining procedure, while paratumor cirrhosis, benign nodular tissue and normal liver tissue would not induce a change in its fluorescence. Compared to the most authoritative clinical method, i.e., haematoxylin and eosin staining, our method has better detection speed and accuracy for intraoperative frozen-section consultation. The results indicated that ZZ-HPB-NC represents an ideal marker for simply, quickly and highly efficiently distinguishing HCC cells from normal liver cells. ZZ-HPB-NC staining is an objective for intraoperative HCC diagnosis with potential clinical applications.

中文翻译：

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六苯基-1,3-丁二烯衍生物：用于肝细胞癌术中病理诊断的新型“开启”快速荧光探针

在这里，我们报告一种新型的荧光探针，六苯基-1,3-丁二烯衍生物（ZZ-HPB-NC），具有聚集诱导发射（AIE）功能，显示出对以下物质的简单，高度选择性，特异性和即时响应肝细胞癌（HCC）。ZZ-HPB-NC的荧光发射可通过简单的染色过程在HCC术中冰冻切片的术中快速开启，而癌旁肝硬化，良性结节组织和正常肝组织不会引起其荧光变化。与最权威的临床方法相比，即，苏木精和曙红染色，我们的方法对于术中冰冻切片咨询具有更好的检测速度和准确性。结果表明，ZZ-HPB-NC是简单，快速和高效区分HCC细胞和正常肝细胞的理想标记。ZZ-HPB-NC染色是术中HCC诊断的目标，具有潜在的临床应用价值。