Nicotiana benthamiana is widely used as a model plant to study plant-pathogen interactions. Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), participate in mediating plant growth, development and response to abiotic stress. However, the members of FLAs in N. benthamiana and their response to plant pathogens are unknown. 38 NbFLAs were identified from a genome-wide study. NbFLAs could be divided into four subclasses, and their gene structure and motif composition were conserved in each subclass. NbFLAs may be regulated by cis-acting elements such as STRE and MBS, and may be the targets of transcription factors like C2H2. Quantitative real time polymerase chain reaction (RT-qPCR) results showed that selected NbFLAs were differentially expressed in different tissues. All of the selected NbFLAs were significantly downregulated following infection by turnip mosaic virus (TuMV) and most of them also by Pseudomonas syringae pv tomato strain DC3000 (Pst DC3000), suggesting possible roles in response to pathogenic infection. This study systematically identified FLAs in N. benthamiana, and indicates their potential roles in response to biotic stress. The identification of NbFLAs will facilitate further studies of their role in plant immunity in N. benthamiana.

中文翻译：

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本塞姆氏烟草中的类Fasciclin阿拉伯半乳聚糖基因家族：响应病原体的全基因组鉴定、分类和表达。


本塞姆氏烟草被广泛用作研究植物与病原体相互作用的模型植物。成束环蛋白样阿拉伯半乳聚糖蛋白 (FLA) 是阿拉伯半乳聚糖蛋白 (AGP) 的一个亚类，参与介导植物生长、发育和对非生物胁迫的响应。然而，本塞姆氏烟草中FLA的成员及其对植物病原体的反应尚不清楚。通过全基因组研究鉴定出 38 种 NbFLA。 NbFLA可分为四个亚类，每个亚类的基因结构和基序组成都是保守的。 NbFLAs 可能受到 STRE 和 MBS 等顺式作用元件的调节，并且可能是 C2H2 等转录因子的靶标。实时定量聚合酶链反应（RT-qPCR）结果表明，选定的 NbFLAs 在不同组织中存在差异表达。所有选定的 NbFLA 在被萝卜花叶病毒 (TuMV) 感染后均显着下调，其中大多数也被丁香假单胞菌番茄菌株 DC3000 (Pst DC3000) 感染，这表明可能在应对病原体感染中发挥作用。这项研究系统地鉴定了本塞姆氏烟草中的 FLA，并指出了它们在应对生物胁迫中的潜在作用。 NbFLA 的鉴定将有助于进一步研究它们在本塞姆氏烟草植物免疫中的作用。