Ultrasensitive analysis of microRNAs with gold nanoparticle-decorated molybdenum disulfide nanohybrid-based multilayer nanoprobes.,Chemical Communications

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Ultrasensitive analysis of microRNAs with gold nanoparticle-decorated molybdenum disulfide nanohybrid-based multilayer nanoprobes.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-07-01 , DOI: 10.1039/d0cc03845h
Shao Su ₁ , Qian Sun , Jianfeng Ma , Dan Zhu , Fei Wang , Jie Chao , Chunhai Fan , Qian Li , Lianhui Wang

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The nanoprobe-based signal amplification strategy is a powerful way to ultrasensitively detect biomolecules. Herein, a gold nanoparticle-decorated molybdenum disulfide (MoS₂–AuNP)-based multilayer nanoprobe (MLNP) was designed for ultrasensitive analysis of microRNA-21 (miRNA-21). The MLNP-amplified electrochemical biosensor exhibited an ultrawide dynamic range (10 aM–1 μM) and an ultralow detection limit (38 aM) for target miRNA-21 analysis. Furthermore, this biosensor can determine miRNA-21 expression in cell lysates of 100 human cervical cancer (HeLa) cells. Our results demonstrate that MoS₂–AuNP nanocomposites have great potential in constructing biosensors for target molecule analysis.

中文翻译：

使用金纳米颗粒修饰的二硫化钼纳米杂化纳米多层探针对microRNA进行超灵敏分析。

基于纳米探针的信号放大策略是超灵敏地检测生物分子的有效方法。本文中，设计了一种金纳米颗粒修饰的二硫化钼（MoS ₂ -AuNP）基多层纳米探针（MLNP），用于microRNA-21（miRNA-21）的超灵敏分析。MLNP放大的电化学生物传感器对目标miRNA-21分析显示出超宽的动态范围（10 aM–1μM）和超低的检测限（38 aM）。此外，该生物传感器可以确定100个人类宫颈癌（HeLa）细胞的细胞裂解物中的miRNA-21表达。我们的结果表明，MoS ₂ -AuNP纳米复合材料在构建用于靶分子分析的生物传感器方面具有巨大潜力。

更新日期：2020-08-06

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