In recent decades, a major shift in the land use pattern has been observed in Dhaka, the capital city of Bangladesh. To understand and model the impact of these land use changes on transportation demand, this study aimed to determine the trip generation rates for six different land use categories adjacent to Mirpur Road in Dhaka. A total of 20 establishments consisting of six land use categories were selected for the collection of data on person trip rates and respective modal share by manual counts and intercept surveys. These data were used to develop vehicular trip generation rates for each land use category in passenger car equivalents as a uniform unit of comparison. Results showed that commercial and healthcare land uses had the highest average and peak-hour trip rates. There was also a significant variation in the share of eight transport mode categories among the trips generated by the land uses. The peak-hour trip generation rates of the study area were found to be different from the values established by the Institute of Transportation Engineers which corresponds to the fact that trip generation depends on a host of factors, such as surrounding land uses, modal share, the economic condition of a region, and so forth, rather than on a single factor inherent to the land use. The findings of this research can help to determine the trip generation impact of new establishments and consequently identify suitable locations to minimize the impact.