Background:

Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment.

Methods:

In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour.

Results:

Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg⁻¹ and 0.3 mg kg⁻¹ doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly.

Conclusions:

Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.

中文翻译：

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使用吲哚菁绿的实时荧光成像评估肿瘤模型小鼠的近红外光免疫疗法的治疗效果。

背景：

近红外光免疫疗法（NIR-PIT）是一种导致肿瘤灌注增加的癌症疗法，这种现象被称为超增强的渗透性和保留作用。目前，NIR-PIT的体内治疗功效在治疗后数天即可观察到，但通过更急性地检测肿瘤内变化，监测将得到改善。荧光成像可在治疗后立即检测到肿瘤灌注增加。

方法：

在第一个实验中，仅使用NIR-PIT或激光疗法治疗携带单侧皮下胁腹肿瘤的无胸腺裸鼠模型。在第二个实验中，只对左侧肿瘤用NIR-PIT治疗患有双侧胁腹肿瘤的小鼠。两组均在治疗后立即静脉内注射不同剂量的吲哚菁绿，并用Shimadzu LIGHTVISION荧光成像系统监测小鼠1小时。

结果：

在处理的小鼠中，在监测的60分钟内，荧光强度的肿瘤与背景之比增加，但在对照小鼠中，无明显变化。1 mg kg ^-1和0.3 mg kg ^-1剂量的肿瘤与背景之比最高。在患有双侧肿瘤的小鼠中，肿瘤与未治疗的肿瘤比率相似地增加。

结论：

NIR-PIT后肿瘤灌注的急性变化可通过实时荧光成像检测。