Maintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genus Mazama endure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the species Mazama americana: 6% glycerol (GLY), 3% ethylene glycol (ETG), and 5% dimethylformamide (DMF). Semen was obtained with the lateral deviation of the penis to an artificial vagina. In the pre-freeze and post-thaw periods, motility, vigor, membrane integrity, acrosome integrity, and sperm cell morphology were evaluated for each of the cryoprotectants. Post-thaw motility was higher when semen was frozen with cryoprotectants GLY and DMF (55.31 ± 7.39 and 55.94 ± 2.77, respectively), compared with the result obtained for ETG (48.13 ± 2.39). For major defects (MaD), a difference was observed between the pre- and post-cryopreservation periods, such that DMF generated a higher number of post-thaw MaD (25.94 ± 5.37). All cryoprotectants were efficient for cryopreservation of M. americana semen, resulting in samples with satisfactory viability after thawing. However, the medium with the cryoprotectants GLY, at a concentration of 6%, and DMF, at a concentration of 5%, were preferable.

中文翻译：

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三种冷冻保护剂在冷冻人工阴道收集的美国马扎马精液中的比较。

保持遗传变异性是濒危物种保护的重要组成部分，因此种质库的建设必不可少。Mazama属的几个物种在其自然栖息地承受着持续的压力，并面临灭绝的威胁。必须研究稀释剂和冷冻保护剂的正确操作和充分性，才能成功地形成这些库。本研究的目的是评估三种不同冷冻保护剂在美洲马扎马物种精子冷冻保存中的效率。：6% 甘油 (GLY)、3% 乙二醇 (ETG) 和 5% 二甲基甲酰胺 (DMF)。精液是通过阴茎侧向偏离人造阴道获得的。在冷冻前和解冻后阶段，评估了每种冷冻保护剂的运动性、活力、膜完整性、顶体完整性和精子细胞形态。与 ETG 获得的结果 (48.13 ± 2.39) 相比，当用冷冻保护剂 GLY 和 DMF 冷冻精液时，解冻后的运动性更高（分别为 55.31 ± 7.39 和 55.94 ± 2.77）。对于主要缺陷 (MaD)，在冷冻前和冷冻后期间观察到差异，因此 DMF 产生了更多的解冻后 MaD (25.94 ± 5.37)。所有冷冻保护剂对美洲木耳的冷冻保存均有效精液，使样品在解冻后具有令人满意的活力。然而，优选含有浓度为 6% 的冷冻保护剂 GLY 和浓度为 5% 的 DMF 的培养基。