Fluorescent Membrane Tension Probes for Super-Resolution Microscopy: Combining Mechanosensitive Cascade Switching with Dynamic-Covalent Ketone Chemistry,Journal of the American Chemical Society

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Fluorescent Membrane Tension Probes for Super-Resolution Microscopy: Combining Mechanosensitive Cascade Switching with Dynamic-Covalent Ketone Chemistry
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2020-07-01 , DOI: 10.1021/jacs.0c04942
José García-Calvo ₁ , Jimmy Maillard ₁ , Ina Fureraj ₁ , Karolina Strakova ₁ , Adai Colom ₁ , Vincent Mercier ₁ , Aurelien Roux ₁ , Eric Vauthey ₁ , Naomi Sakai ₁ , Alexandre Fürstenberg ₁ , Stefan Matile ₁

Affiliation

We report the design, synthesis, and evaluation of fluorescent flipper probes for single-molecule super-resolution imaging of membrane tension in living cells. Reversible switching from bright-state ketones to dark-state hydrates, hemiacetals, and hemithioacetals is demonstrated for twisted and planarized mechanophores in solution and membranes. Broadband femtosecond fluorescence up-conversion spectroscopy evinces ultrafast chalcogen-bonding cascade switching in the excited state in solution. According to fluorescence lifetime imaging microscopy, the new flippers image membrane tension in live cells with record red shifts and photostability. Single-molecule localization microscopy with the new tension probes resolves membranes well below the diffraction limit.

中文翻译：

用于超分辨率显微镜的荧光膜张力探针：将机械敏感级联开关与动态共价酮化学相结合

我们报告了用于活细胞膜张力单分子超分辨率成像的荧光鳍状探针的设计、合成和评估。从亮态酮到暗态水合物、半缩醛和半硫缩醛的可逆转换被证明用于溶液和膜中的扭曲和平面化机械团。宽带飞秒荧光上转换光谱表明溶液中激发态的超快硫属元素键联级联切换。根据荧光寿命成像显微镜，新的鳍状肢对活细胞中的膜张力进行成像，并具有记录的红移和光稳定性。带有新型张力探针的单分子定位显微镜可解析远低于衍射极限的膜。

更新日期：2020-07-01

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