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Immunomodulatory Effect of Laccase/Caffeic Acid and Transglutaminase in Alleviating Shrimp Tropomyosin (Met e 1) Allergenicity.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-01 , DOI: 10.1021/acs.jafc.0c02366 Ishfaq Ahmed 1 , Hong Lin 1 , Lili Xu 1 , Shuang Li 2 , Joana Costa 3 , Isabel Mafra 3 , Guanzhi Chen 4 , Xiang Gao 4 , Zhenxing Li 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-01 , DOI: 10.1021/acs.jafc.0c02366 Ishfaq Ahmed 1 , Hong Lin 1 , Lili Xu 1 , Shuang Li 2 , Joana Costa 3 , Isabel Mafra 3 , Guanzhi Chen 4 , Xiang Gao 4 , Zhenxing Li 1
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This work aimed to investigate the effect of enzymatic cross-linking on the allergenic potential of shrimp tropomyosin (TM), Met e 1. The cross-linked TM with laccase (CL), laccase/caffeic acid (CLC and CLC+), and transglutaminase (CTG and CTG+) formed macromolecules and altered the allergen conformation. The IgG/IgE-binding potentials of the cross-linked TM were reduced as confirmed by Western blotting and ELISA. Enzymatic cross-linking improved the gastrointestinal digestibility and induced a lower level of degranulation in RBL-2H3 and KU812 cells. Moreover, cross-linked TM decreased anaphylactic symptoms, as well as reduced the serum levels of IgG1, IgE, histamine, tryptase, and mMCP-1. In spleen cells, CLC+ and CTG+ downregulated the Th2-related cytokines and upregulated IFN-γ and IL-10. These findings revealed that CTG+ has shown more potential than CLC+ in mitigating the allergenicity of TM by influencing the conformational structure, enhancing the digestibility, decreasing the cellular degranulation process, and positively modulating the Th1/Th2 immunobalance.
中文翻译：
漆酶/咖啡酸和转谷氨酰胺酶对减轻虾原肌球蛋白（Met e 1）的致敏性的免疫调节作用。
这项工作旨在研究酶促交联对虾原肌球蛋白（TM）变应原潜力的影响。1。与漆酶（CL），漆酶/咖啡酸（CLC和CLC +）交联的TM转谷氨酰胺酶（CTG和CTG +）形成大分子并改变了过敏原构象。通过Western印迹和ELISA证实，交联TM的IgG / IgE结合电位降低。酶促交联改善了肠胃的消化率，并诱导了RBL-2H3和KU812细胞中较低的脱粒水平。此外，交联的TM可减少过敏性症状，并降低IgG1，IgE，组胺，类胰蛋白酶和mMCP-1的血清水平。在脾细胞中，CLC +和CTG +下调Th2相关细胞因子，上调IFN-γ和IL-10。这些发现表明，CTG +通过影响构象结构，增强消化率，减少细胞脱粒过程以及正调节Th1 / Th2免疫平衡，在缓解TM变应原性方面显示出比CLC +更多的潜力。
更新日期：2020-07-22
中文翻译：
漆酶/咖啡酸和转谷氨酰胺酶对减轻虾原肌球蛋白（Met e 1）的致敏性的免疫调节作用。
这项工作旨在研究酶促交联对虾原肌球蛋白（TM）变应原潜力的影响。1。与漆酶（CL），漆酶/咖啡酸（CLC和CLC +）交联的TM转谷氨酰胺酶（CTG和CTG +）形成大分子并改变了过敏原构象。通过Western印迹和ELISA证实，交联TM的IgG / IgE结合电位降低。酶促交联改善了肠胃的消化率，并诱导了RBL-2H3和KU812细胞中较低的脱粒水平。此外，交联的TM可减少过敏性症状，并降低IgG1，IgE，组胺，类胰蛋白酶和mMCP-1的血清水平。在脾细胞中，CLC +和CTG +下调Th2相关细胞因子，上调IFN-γ和IL-10。这些发现表明，CTG +通过影响构象结构，增强消化率，减少细胞脱粒过程以及正调节Th1 / Th2免疫平衡，在缓解TM变应原性方面显示出比CLC +更多的潜力。
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