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Characterization and Semiquantitative Analysis of Novel Ultratrace C10-24 Monounsaturated Fatty Acid in Bovine Milkfat by Solvent-Mediated Covalent Adduct Chemical Ionization (CACI) MS/MS.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-01 , DOI: 10.1021/acs.jafc.0c03031 Dong Hao Wang 1, 2 , Zhen Wang 1, 2 , Raymond Chen 1 , J Thomas Brenna 1, 2, 3
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-07-01 , DOI: 10.1021/acs.jafc.0c03031 Dong Hao Wang 1, 2 , Zhen Wang 1, 2 , Raymond Chen 1 , J Thomas Brenna 1, 2, 3
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The net action of ruminal bacteria and endogenous bovine enzymes are responsible for cow’s milk having the most complex fatty acid profiles among common foods. About 40 monounsaturated fatty acids below 1.5% w/w are known. Analysis of trace and ultratrace fatty acids is a challenge to the highest resolution chromatography even with prior fractionation. We employ solvent-mediated covalent adduct chemical ionization (CACI) tandem mass spectrometry (MS/MS) to enable rapid, unambiguous identification of unsaturated fatty acid methyl esters (FAME) at high sensitivity. Fifty-four monounsaturated fatty acids (C10–24) were completely characterized, with the discovery of 15 novel fatty acids including nine at ultratrace levels 10–100 ppm, g/106 g fatty acids (lowest concentration 19:1n-6 (10 ± 11 ppm, w/w (0.001%, w/w))). Ultratrace monoenes were typically odd chain lengths and all analyzed in a single 20 min analysis. These data establish the abundance of 15 new monoene fatty acids in bovine milkfat and a strategy for rapid unambiguous analysis of ultratrace monounsaturated fatty acids.
中文翻译：
溶剂介导的共价加合物化学电离（MSCI）对牛乳中新型超痕量C10-24单不饱和脂肪酸的表征和半定量分析。
瘤胃细菌和内源性牛酶的净作用是导致普通食品中具有最复杂脂肪酸特征的牛奶的原因。已知低于1.5％w / w的约40种单不饱和脂肪酸。痕量和超痕量脂肪酸的分析是即使采用事先分级分离对最高分辨率色谱法的挑战。我们采用溶剂介导的共价加合物化学电离（CACI）串联质谱（MS / MS），能够以高灵敏度快速，明确地鉴定不饱和脂肪酸甲酯（FAME）。54种单不饱和脂肪酸（C 10-24）的特征得到了充分表征，发现了15种新型脂肪酸，其中9种超痕量水平为10-100 ppm，g / 10 6 g脂肪酸（最低浓度19：1）n -6（10±11 ppm，w / w（0.001％，w / w）））。超痕量单烯通常为奇数链长，所有分析均在20分钟内完成。这些数据建立了牛乳中15种新的单烯脂肪酸的丰度，并提出了一种用于超痕量单不饱和脂肪酸的快速明确分析的策略。
更新日期：2020-07-15
中文翻译：
溶剂介导的共价加合物化学电离（MSCI）对牛乳中新型超痕量C10-24单不饱和脂肪酸的表征和半定量分析。
瘤胃细菌和内源性牛酶的净作用是导致普通食品中具有最复杂脂肪酸特征的牛奶的原因。已知低于1.5％w / w的约40种单不饱和脂肪酸。痕量和超痕量脂肪酸的分析是即使采用事先分级分离对最高分辨率色谱法的挑战。我们采用溶剂介导的共价加合物化学电离（CACI）串联质谱（MS / MS），能够以高灵敏度快速，明确地鉴定不饱和脂肪酸甲酯（FAME）。54种单不饱和脂肪酸（C 10-24）的特征得到了充分表征，发现了15种新型脂肪酸，其中9种超痕量水平为10-100 ppm，g / 10 6 g脂肪酸（最低浓度19：1）n -6（10±11 ppm，w / w（0.001％，w / w）））。超痕量单烯通常为奇数链长，所有分析均在20分钟内完成。这些数据建立了牛乳中15种新的单烯脂肪酸的丰度，并提出了一种用于超痕量单不饱和脂肪酸的快速明确分析的策略。
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