To explore the molecular mechanism of the effect of Bacillus cereus PAS38 on the immunity of broilers, sixty 7-day-old broilers were divided into two groups with three replicates. The control group was fed with basal diet, and the treatment group was fed with basal diet containing Bacillus cereus PAS38 1×10⁶ CFU/g. Thymus and bursa of fabricius were taken from two groups of broilers at the age of 42 days, total RNA was extracted, differential gene library was constructed by SSH technology, and immune-related differential genes were screened. Then, we used siRNA to interfere with the expression of some differential genes in the original generation lymphocytes of broiler blood to detect the change of cytokines mRNA expression level. A total of 42 immune-related differentially expressed genes were screened, including 22 up-regulated genes and 20 down-regulated genes. When 7 differentially up-regulated genes associated with enhanced immune function were interfered with in lymphocytes, some immune-promoting cytokines were down-regulated. These results showed that Bacillus cereus PAS38 might up-regulate the expression of JCHAIN, PRDX1, CD3E, CDK6 and other genes in immune organs of broilers, thereby affecting the development of immune organs, the expression of various cytokines and the transduction of immune signals, improving the immune capacity of broilers.

为了探讨蜡状芽孢杆菌PAS38对肉鸡免疫的影响的分子机制，将60只7日龄的肉鸡分为两组，每组重复三次。对照组饲喂基础饲料，治疗组饲喂蜡状芽孢杆菌PAS38 1×10 ⁶CFU /克。从两组42岁的肉鸡中提取胸腺和法氏囊，提取总RNA，通过SSH技术构建差异基因文库，并筛选免疫相关的差异基因。然后，我们使用siRNA干扰肉鸡血液原始代淋巴细胞中某些差异基因的表达，以检测细胞因子mRNA表达水平的变化。共筛选了42个免疫相关的差异表达基因，包括22个上调基因和20个下调基因。当7种与免疫功能增强相关的差异上调基因受到干扰时，一些免疫促进细胞因子被下调。这些结果表明蜡状芽孢杆菌PAS38可能上调的表达JCHAIN，PRDX1，CD3E，CDK6和其他基因在肉鸡的免疫器官，进而影响免疫器官的发育，多种细胞因子的表达和免疫信号的传导，提高肉鸡的免疫能力。