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Cecal Butyrate (Not Propionate) Was Connected with Metabolism-Related Chemicals of Mice, Based on the Different Effects of the Two Inonotus obliquus Extracts on Obesity and Their Mechanisms.
ACS Omega ( IF 3.7 ) Pub Date : 2020-06-30 , DOI: 10.1021/acsomega.0c01566 Jian Yu 1 , Jun-Yan Xiang 1 , Hongyu Xiang 1, 2, 3 , Qiuhong Xie 1, 2, 3
ACS Omega ( IF 3.7 ) Pub Date : 2020-06-30 , DOI: 10.1021/acsomega.0c01566 Jian Yu 1 , Jun-Yan Xiang 1 , Hongyu Xiang 1, 2, 3 , Qiuhong Xie 1, 2, 3
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Obesity is a metabolic disease and causes significant changes in host and gut microbial metabolite levels. However, little research has been done on the relationship between host and gut microbial metabolites. Thus, this study investigated the connection of the chemicals, based on the different effects of two Inonotus obliquus extracts on high-fat-diet-induced mice and their mechanisms. In this study, C57BL6/J mice fed with a high-fat diet were given I. obliquus ethanol extract (IOE) and polysaccharide (IOP). 1H NMR-based metabolomics, 16S rRNA sequencing, and real-time reverse transcription polymerase chain reaction (RT-PCR) were used to detect metabolites, cecal microbes, and expressions of genes in liver. IOE and IOP effectively improved the obesity of mice, including the adjustment of body weight gain, energy intake, energy efficiency, liver glucose metabolism and triglyceride metabolism, tricarboxylic acid (TCA) cycle, and degradation of three major nutrients (carbohydrate, lipid, and protein). IOE significantly increased cecal propionate based on Bacteroides and Akkermansia, thereby inhibiting energy intake and fat accumulation in mice. IOP remarkably improved the level of cecal butyrate by Lactobacillus and the Bacteroidales S24-7 group, resulting in increased energy consumption, and fat degradation by regulating the TCA cycle of the host. Two extracts containing different bioactive substances of I. obliquus improved obesity in mice through different effects on production of cecal microbial metabolites. Moreover, cecal butyrate (not propionate) was connected with chemicals of mice, including four metabolites of the TCA cycle and other metabolism-related chemicals.
中文翻译：
基于两种桦褐孔菌提取物对肥胖的不同作用及其机制，将盲肠丁酸盐（不是丙酸盐）与小鼠代谢相关的化学物质联系起来。
肥胖是一种代谢疾病，会导致宿主和肠道微生物代谢物水平发生显着变化。然而，关于宿主和肠道微生物代谢物之间的关系的研究很少。因此，本研究基于两种桦褐孔菌提取物对高脂肪饮食诱导的小鼠的不同影响及其机制，调查了化学物质之间的联系。在这项研究中，给喂食高脂肪饮食的 C57BL6/J 小鼠喂食斜花乙醇提取物 (IOE) 和多糖 (IOP)。基于1 H NMR 的代谢组学、16S rRNA 测序和实时逆转录聚合酶链反应 (RT-PCR) 用于检测代谢物、盲肠微生物和肝脏中基因的表达。 IOE和IOP有效改善了小鼠的肥胖，包括调整体重增加、能量摄入、能量效率、肝脏葡萄糖代谢和甘油三酯代谢、三羧酸（TCA）循环以及三大营养素（碳水化合物、脂质和代谢物）的降解。蛋白质）。 IOE显着增加基于拟杆菌和阿克曼氏菌的盲肠丙酸，从而抑制小鼠的能量摄入和脂肪积累。 IOP显着提高了乳酸杆菌和拟杆菌S24-7群的盲肠丁酸水平，导致能量消耗增加，并通过调节宿主的TCA循环来降解脂肪。两种含有不同生物活性物质的斜叶草提取物通过对盲肠微生物代谢产物产生的不同影响改善了小鼠的肥胖。 此外，盲肠丁酸盐（不是丙酸盐）与小鼠的化学物质有关，包括TCA循环的四种代谢物和其他代谢相关的化学物质。
更新日期：2020-07-14
中文翻译：
基于两种桦褐孔菌提取物对肥胖的不同作用及其机制，将盲肠丁酸盐（不是丙酸盐）与小鼠代谢相关的化学物质联系起来。
肥胖是一种代谢疾病，会导致宿主和肠道微生物代谢物水平发生显着变化。然而，关于宿主和肠道微生物代谢物之间的关系的研究很少。因此，本研究基于两种桦褐孔菌提取物对高脂肪饮食诱导的小鼠的不同影响及其机制，调查了化学物质之间的联系。在这项研究中，给喂食高脂肪饮食的 C57BL6/J 小鼠喂食斜花乙醇提取物 (IOE) 和多糖 (IOP)。基于1 H NMR 的代谢组学、16S rRNA 测序和实时逆转录聚合酶链反应 (RT-PCR) 用于检测代谢物、盲肠微生物和肝脏中基因的表达。 IOE和IOP有效改善了小鼠的肥胖，包括调整体重增加、能量摄入、能量效率、肝脏葡萄糖代谢和甘油三酯代谢、三羧酸（TCA）循环以及三大营养素（碳水化合物、脂质和代谢物）的降解。蛋白质）。 IOE显着增加基于拟杆菌和阿克曼氏菌的盲肠丙酸，从而抑制小鼠的能量摄入和脂肪积累。 IOP显着提高了乳酸杆菌和拟杆菌S24-7群的盲肠丁酸水平，导致能量消耗增加，并通过调节宿主的TCA循环来降解脂肪。两种含有不同生物活性物质的斜叶草提取物通过对盲肠微生物代谢产物产生的不同影响改善了小鼠的肥胖。 此外，盲肠丁酸盐（不是丙酸盐）与小鼠的化学物质有关，包括TCA循环的四种代谢物和其他代谢相关的化学物质。
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