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Microfluidic accumulation assay to quantify the attachment of the marine bacterium Cobetia marina on fouling-release coatings.
Biointerphases ( IF 2.1 ) Pub Date : 2020-06-25 , DOI: 10.1116/6.0000240
Jana Schwarze 1 , Robin Wanka 1 , Axel Rosenhahn 1
Affiliation  

Testing the adhesion of marine biofilm formers on bioresistant coatings is important to determine their fouling-release and antifouling properties. A dynamic attachment assay for the marine bacterium Cobetia marina (C. marina) was developed to test the adhesion on coatings and bioresistant surfaces. With well-defined culture conditions, the reproducibility of the microfluidic accumulation experiments with C. marina was verified using self-assembling monolayers as model surfaces. The assay discriminated the attachment of C. marina on four different surfaces with different wettability and protein resistances. In addition to these benchmark experiments on self-assembled monolayers, the adhesion of C. marina on polyglycerol coatings with different thicknesses was investigated.

中文翻译:

微流体累积测定法可量化海洋细菌Cobetia marina在污垢释放涂层上的附着。

测试海洋生物成膜剂在抗生物涂层上的附着力对于确定其污垢释放和防污性能很重要。开发了一种用于海洋细菌Cobetia marinaC. marina)的动态附着测定法,以测试在涂层和生物抗性表面上的附着力。在明确定义的培养条件下,使用自组装单层作为模型表面验证了滨海假丝酵母微流体蓄积实验的可重复性。该测定法区分了滨海假丝酵母在四个具有不同润湿性和蛋白质抗性的不同表面上的附着。除了这些关于自组装单层的基准实验外,滨海梭菌的粘附力 研究了不同厚度的聚甘油涂料的涂层性能。
更新日期:2020-07-01
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