Aedes mosquitoes vector many viruses with divergent characteristics, yet the criteria needed for a virus to be vectored by an arthropod remain unknown. The intracellular cholesterol transporter protein Niemann–Pick C1 (NPC1) has been identified as the necessary entry receptor for filoviruses such as Ebola and Marburg viruses. While homologues of NPC1 are observed in mosquitoes, currently no filovirus has been identified as circulating in mosquitoes. This work aimed at increasing the understanding of the mosquito vector by examining the capability of a virus to gain the ability to enter mosquito cells. We developed a model system of Aedes cells expressing human NPC1 (hNPC1) and attempted to infect these cells with recombinant vesicular stomatitis virus expressing the Ebola virus glycoprotein. As compared to the control cells, no significant increase in infection was observed in cells expressing hNPC1, demonstrating that the expression of human NPC1 alone is not sufficient to support filovirus infection, and that host factors other than NPC1 determine filovirus susceptibility of mosquito cells.

中文翻译：

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检查 Niemann-Pick C1 蛋白在伊蚊对丝状病毒的许可中的作用。

伊蚊携带许多具有不同特征的病毒，但节肢动物携带病毒所需的标准仍然未知。细胞内胆固醇转运蛋白 Niemann-Pick C1 (NPC1) 已被确定为埃博拉病毒和马尔堡病毒等丝状病毒的必要进入受体。虽然在蚊子中观察到 NPC1 的同源物，但目前尚未发现丝状病毒在蚊子中传播。这项工作旨在通过检查病毒获得进入蚊子细胞的能力来增加对蚊子载体的了解。我们开发了一个伊蚊模型系统表达人 NPC1 (hNPC1) 的细胞并试图用表达埃博拉病毒糖蛋白的重组水泡性口炎病毒感染这些细胞。与对照细胞相比，在表达 hNPC1 的细胞中没有观察到感染的显着增加，这表明仅人类 NPC1 的表达不足以支持丝状病毒感染，并且 NPC1 以外的宿主因素决定了蚊子细胞对丝状病毒的易感性。