The purpose of this study was to explore the application of glucose to improve embryo development potential when fertilization is delayed in mice. After recovery, mouse oocytes were cultured alone for 6 h before fertilization in three fertilization media: G-IVF PLUS, G-IVF PLUS with 5 mM and 10 mM glucose. G-IVF PLUS group was used as the control group. Then, in vitro fertilization (IVF) was performed and blastocysts were transferred at Day 4. To compare the effects of different glucose concentrations on embryo development and birth outcomes, conventional IVF and embryo transfer were carried out in G-IVF PLUS, G-IVF PLUS with 5 mM and 10 mM glucose. The results indicated that G-IVF PLUS with 5 mM glucose significantly increased blastocyst rate (p < 0.05) and birth rate (p < 0.05) when fertilization was delayed 6 h compared with G-IVF PLUS groups. In conventional IVF without delayed fertilization, embryo development was not significantly affected by G-IVF PLUS with 5 mM or 10 mM glucose. There were no significant differences in terms of birth rate, fetal weight, crown-rump length, tail length and birth defect rate among the three groups. In conclusion, 5 mM glucose could significantly improve embryo developmental potential and birth outcomes when fertilization was delayed 6 h and did not have adverse effects on embryo quality and birth outcomes for normal IVF. It might have a good prospect of clinical application in assisted reproductive technology (ART).

Abbreviations: ART: assisted reproductive technology; IVF: in vitro fertilization; ICSI: intracytoplasmic sperm injection; TFF: total fertilization failure; TESA: testicular sperm aspiration.

本研究的目的是探索葡萄糖在小鼠受精延迟时提高胚胎发育潜力的应用。恢复后，小鼠卵母细胞单独培养 6 小时，然后在三种受精培养基中受精：G-IVF PLUS、含有 5 mM 和 10 mM 葡萄糖的 G-IVF PLUS。G-IVF PLUS组作为对照组。然后，进行体外受精(IVF) 并在第 4 天移植囊胚。 为了比较不同葡萄糖浓度对胚胎发育和出生结果的影响，在 G-IVF PLUS、G-IVF 中进行常规 IVF 和胚胎移植加上 5 mM 和 10 mM 葡萄糖。结果表明，含 5 mM 葡萄糖的 G-IVF PLUS 显着增加了囊胚率 ( p < 0.05) 和出生率 ( p< 0.05) 与 G-IVF PLUS 组相比，受精延迟 6 小时。在没有延迟受精的常规试管婴儿中，含 5 mM 或 10 mM 葡萄糖的 G-IVF PLUS 对胚胎发育没有显着影响。三组在出生率、胎儿体重、头臀长、尾长和出生缺陷率方面无显着差异。总之，当受精延迟 6 小时时，5 mM 葡萄糖可以显着改善胚胎发育潜力和出生结果，并且对正常 IVF 的胚胎质量和出生结果没有不利影响。在辅助生殖技术（ART）中可能具有良好的临床应用前景。

缩写：ART：辅助生殖技术；试管婴儿：体外受精；ICSI：胞浆内单精子注射；TFF：受精总失败；TESA：睾丸精子抽吸术。