Context: Astragali Radix (AR) and Angelica sinensis Radix (ASR) combinations are used to treat cardiovascular disorders.Objectives: This study investigates the protective effects of different compatibility proportions of AR and ASR on cardiac dysfunction in a C57BL/6 mouse model of myocardial infarction (MI).Materials and methods: MI mice were induced by ligation of the left coronary artery and divided into six groups: sham, vehicle, 10 mg/kg/d simvastatin and combinations of AR and ASR at different ratios, including 1:1 (AR 2.5 g/kg + ASR 2.5 g/kg), 3:1 (AR 3.75 g/kg + ASR 1.25 g/kg) and 5:1 (AR 4.17 g/kg + ASR 0.83 g/kg). Both AR-ASR combinations and simvastatin were dissolved in saline solution and given daily by gavage. The left ventricle function, infarct size, heart tissue injury, apoptosis of cardiomyocytes, leukocyte infiltrates, capillary density and expression of cleaved caspase-3, cleaved caspase-9, Bcl-2, Bax, Bad, IL-1β, IL-6, VEGF, p-Akt and p-eNOS were analysed.Results: Different combinations of AR and ASR improve cardiac function and reduce infarct size (61.15% vs. 39.3%, 42.65% and 45.5%) and tissue injury through different mechanisms. When AR was combined with ASR at ratio of 1:1, the inflammation and cardiomyocyte apoptosis were suppressed (p < 0.05, p < 0.01). The combination ratio of 3:1 exerted effect in promoting angiogensis (p < 0.05). In the combination of AR and ASR at 5:1 ratio, angiogenesis was significantly improved (p < 0.01) and the apoptosis was inhibited (p < 0.05).Conclusions: Our results reflect the regulation of multiple targets and links in herb pairs and provide an important basis for the use of AR and ASR combinations in the treatment of MI.

中文翻译：

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黄芪、当归联药不同配伍比例对心肌梗死损伤的保护作用


背景：黄芪（AR）和当归（ASR）组合用于治疗心血管疾病。 目的：本研究探讨不同配伍比例的 AR 和 ASR 对 C57BL/6 小鼠心肌模型心功能不全的保护作用材料和方法：通过结扎左冠状动脉来诱导MI小鼠，并分为六组：假手术组、媒介物组、10mg/kg/d辛伐他汀组以及不同比例的AR和ASR组合，其中1： 1（AR 2.5 g/kg + ASR 2.5 g/kg）、3:1（AR 3.75 g/kg + ASR 1.25 g/kg）和 5:1（AR 4.17 g/kg + ASR 0.83 g/kg）。 AR-ASR组合和辛伐他汀均溶解在盐水溶液中并每天通过管饲给药。左心室功能、梗死面积、心脏组织损伤、心肌细胞凋亡、白细胞浸润、毛细血管密度及cleaved caspase-3、cleaved caspase-9、Bcl-2、Bax、Bad、IL-1β、IL-6、分析 VEGF、p-Akt 和 p-eNOS。 结果：AR 和 ASR 的不同组合可通过不同机制改善心脏功能并减少梗塞面积（61.15% vs. 39.3%、42.65% 和 45.5%）和组织损伤。当 AR 与 ASR 以 1:1 的比例联合使用时，炎症和心肌细胞凋亡受到抑制 (p < 0.05，p < 0.01)。 3:1的组合比例发挥了促进血管生成的作用(p< 0.05)。 AR 和 ASR 以 5:1 的比例组合时，血管生成显着改善 (p < 0.01)，细胞凋亡受到抑制 (p < 0.05)。 结论：我们的结果反映了草药对中多个靶点和环节的调节为AR和ASR联合治疗MI提供重要依据。