ABSTRACT

Backgroung

Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported. However, limited information is available on the persistence and excretion of HHV-6 and HHV-7 during and after transplant.

Methods

32 renal transplant recipients were followed up before (T1) and after transplant (T2 and T3) and viral replication (via assessment of mRNA) in oral fluid samples investigated. Roseolovirus DNA was detected and quantified via multiplex qPCR. For evaluation of mRNA replication, positive samples were subjected to nested RT-PCR.

Results

Viral replication of HHV-7 was significantly increased during T3 (72.9%), compared to the pre-transplant period T1 (25%; McNemar Test, p= 0.001). Analysis of the viral replicative to quantitative ratio disclosed ahigher number of DNA copies (>10⁶) in positive cases of replication (p < 0.001). Astrong positive correlation (Spearman correlation coefficient = 0.781; p< 0.001) was evident between viral quantities of Roseoloviruses.

Conclusion

Our findings consistently suggest that the salivary gland is an important site of active and persistent infection by roseoloviruses. In view of the increasing problem of Roseoloviruses, pre- and post-transplantation, viral surveillance and monitoring of active replication are pivotal steps for effective screening and treatment of renal transplant patients.

中文翻译：

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对肾移植受者中人类 β 疱疹病毒 6 型和 7 型口腔脱落的纵向研究显示其复制活跃。

 抽象的

 背景

据报道，玫瑰病毒在唾液腺中潜伏和持续存在，在肾移植后经常被重新激活以引起感染。然而，关于移植期间和移植后 HHV-6 和 HHV-7 的持久性和排泄的信息有限。

 方法

32 名肾移植受者在移植前（T1）和移植后（T2 和 T3）进行了随访，并研究了口腔液样本中的病毒复制（通过 mRNA 评估）。通过多重 qPCR 检测和定量玫瑰病毒 DNA。为了评估 mRNA 复制，对阳性样品进行巢式 RT-PCR。

 结果

与移植前 T1 期（25%；麦克尼马尔检验，p=0.001）相比，HHV-7 的病毒复制在 T3 期间显着增加（72.9%）。病毒复制与定量比率的分析表明，在复制阳性的情况下，DNA 拷贝数较高（>10 ⁶ ）（p < 0.001）。玫瑰病毒的病毒数量之间存在明显的强正相关性（斯皮尔曼相关系数 = 0.781；p < 0.001）。

 结论

我们的研究结果一致表明唾液腺是玫瑰病毒活跃和持续感染的重要部位。鉴于玫瑰病毒问题日益严重，移植前和移植后的病毒监测和活跃复制监测是有效筛查和治疗肾移植患者的关键步骤。